DNA polymerase binding kits contain sequencing polymerase and reagents to prepare SMRTbell libraries for long-read sequencing on the PacBio Sequel System.
Binding Kit Consumables
Sequel Binding and Internal Control Kit 3.0PN: 101-626-600
Optimized reagents for binding SMRTbell template libraries to polymerase for DNA sequencing on the Sequel System.
The Sequel Binding and Internal Control Kit 3.0 includes the latest binding kit, Sequel Binding Kit 3.0 (PN 101-500-400) and Sequel DNA Internal Control Complex 3.0 (PN 101-500-300). Together, these reagents are used to prepare polymerase-bound SMRTbell templates for loading onto the Sequel System. Sequel Polymerase 3.0 should be used only with Sequel Sequencing Kit 3.0 (PNs: 101-597-800/900). When combined with the Sequel Sequencing Kit 3.0, generate up to 20 Gb for large-insert libraries and more for other applications.The Sequel DNA Internal Control Complex 3.0 contains a fixed template of 2 kb length, bound to Sequel DNA Polymerase 3.0 for use as an internal sequencing control on the Sequel System.
Reagent quantities support a minimum of 24 binding reactions and up to 70 SMRT Cell runs per binding reaction, depending on sample insert size, application, and concentration.
Note: Sequel Internal Control Complex 3.0 (PN: 101-500-300) also sold separately.
Clean Up Kit Consumables
Loading Clean Up Bead KitPN: 100-715-300
Bead-based method for removing excess polymerase and primers from the DNA polymerase binding reaction prior to diffusion loading onto the Sequel System.
The Loading Clean Up Bead Kit contains reagents to remove excess polymerase from a polymerase binding reaction. Used for clean up of binding reactions that will be diffusion loaded on the Sequel System.
Reagent quantities support 10 clean up reactions.
MagBead Kit v2PN: 100-676-500
Magnetic beads for capturing the DNA polymerase and template complex for MagBead loading onto the Sequel System.
The MagBead Kit v2 contains reagent supplies to capture DNA polymerase and template complex for loading onto the PacBio Systems. Kit components include: MagBeads (PN:100-125-900), MagBead Binding Buffer Kit v2 (PN: 101-046-400) and MagBead Wash Buffer Kit v2 (PN:101-046-500).
Reagent quantities support 8 reactions.
Note: Kit components also sold separately.
Find information on Binding and Cleanup Kits for the PacBio RS II.