Expand your multiplexing efforts
PacBio Systems provide the ability to sequence multiple samples on the same SMRT Cell to increase throughput and enhance the efficiency of your research.
For information on barcoding options including protocols and analysis with:
- SMRT Link v6.0.0, please refer to the information in the Barcoding Overview.
- SMRT Link v5.1.0, please refer to the information in the Barcoding Overview.
- SMRT Link v5.0.1, please refer to the information in the Barcoding Overview.
Sequence files (compressed FASTA) with PacBio barcodes for use on the Sequel System and
the PacBio RS II:
- 16 barcodes for use with Barcoded Adapters v2
- 96 barcodes for use with Barcoded Primers
- 384 barcodes for use with Barcoded Primers
Sequence files (compressed FASTA) with PacBio barcodes for use on the PacBio RS II:
SMRT Link v5.1.0 and later
- 96 barcodes for use with Barcoded Primers
- 384 barcodes for use with Barcoded Primers
SMRT Link v5.0.1 and earlier
- 96 barcodes for use with the Barcoded Universal F/R Primers Plate – 96 (PacBio Product
Part Number: 100-466-100) - 96 barcodes for use with the Barcoded Adapter Complete Prep Kit – 96 (PacBio Product
Part Number: 100-514-900)
Map of the old PacBio RS II barcode names to the new Sequel System and PacBio RS II
Note: The Iso-Seq application supports a different multiplexing strategy. For details about barcoding samples for Iso-Seq analysis, see Barcoding Samples for Isoform Sequencing (Iso-Seq Analysis).
Barcode oligo ordering sheets:
- Asymmetric Barcodes Ordering Sheet for 96 barcodes
- Asymmetric Barcodes Ordering Sheet for 384 barcodes
- Symmetric Barcoded Adapters Ordering Sheet for 384 barcodes (Compatible with Sequel System and PacBio RS II)
- Symmetric Adapters Barcoded Universal Primers Ordering Sheet for 384 barcodes, Forward and Reverse (Compatible with Sequel System and PacBio RS II)
- Symmetric Barcodes Ordering Sheet for 16 Barcoded Adapters v2 (for use with microbial multiplexing with the Sequel System)
Sample prep protocols for multiplexed experiments:
- Procedure & Checklist – Preparing Multiplexed Microbial SMRTbell Libraries for the PacBio Sequel System
- Analysis Procedure: Multiplexed Microbial Assembly with SMRT Link v5.1.0
- Multiplexing using Barcoded Universal Primers for amplicon sequencing (published July 20, 2015)
- Multiplexing using Barcoded Adapters for amplicon sequencing (published April 26, 2017)