December 14, 2023
Do my primers need to be 5′ phosphorylated for amplicon sequencing?
Not necessarily because of the phosphorylation step during library prep.
Product Line: Sequel system
December 14, 2023
What are my options for barcoding samples?
You can find all the resources for barcoding all types of samples on the multiplexing page
December 13, 2023
Can I multiplex more than 384 amplicon samples?
Yes, it is possible to make thousands of asymmetrical combinations with our barcodes. Though we have not validated all of them ourselves, customers have demonstrated this works. Please reach out…
December 13, 2023
What types of genomic variants can be detected from PacBio HiFi data?
PacBio HiFi reads can detect single-nucleotide variants (SNVs), small indels (<20 bp in length) and structural variants (SVs), such as insertions, deletions, duplications, inversions, translocations, short tandem repeats (STRs), and…
December 13, 2023
How much DNA or cDNA do I need to do HiFi sequencing?
For genomic or metagenomic DNA, see page 1 of the following: Procedure & checklist – Preparing whole genome and metagenome libraries using SMRTbell prep kit 3.0 For 16S, see page…
May 19, 2022
Procedure & checklist — Amplification of bacterial full-length 16S rRNA gene with barcoded primers
November 15, 2021
Procedure & Checklist — Preparing 10 kb Library Using SMRTbell Express Template Prep Kit 2.0 for Metagenomics Shotgun Sequencing
November 15, 2021
Procedure & Checklist — Preparing HiFi Libraries from Low DNA Input Using SMRTbell Express Template Prep Kit 2.0
November 15, 2021
Procedure & Checklist — Preparing SMRTbell Libraries using PacBio Barcoded Overhang Adapters for Multiplexing Amplicons
November 15, 2021