December 14, 2023
Can PacBio sequence my samples?
No. PacBio does not accept samples for sequencing. See the list of our global certified service providers (CSPs) by geographic region.
Product Line: Sequel II system
December 14, 2023
Should I do PCR or probe-based hybridization for my targeted sequencing project?
Typically, customers with many samples find it cost-effective to design a probe panel. View the Application brief – HiFi target enrichment best practices or refer to our partnership with Twist…
December 13, 2023
Which protocol do I need to follow to multiplex microbial libraries for WGS?
If you are planning to barcode multiple microbial genomes and sequence them on a SMRT Cell, we recommend using our most recent chemistry and library preparation version, which is described…
December 13, 2023
Where can I find the most recent protocols for long-read sequencing?
You can find the protocols (procedure & checklists) on the protocols page (filtered view from featured documentation).
December 13, 2023
How does SMRT sequencing work? What is a HiFi read?
HiFi reads are produced using circular consensus sequencing (CCS) mode on PacBio long-read systems. HiFi reads provide base-level resolution with 99.9% single-molecule read accuracy. HiFi reads can be used across…
December 13, 2023
Can I use PacBio sequencing systems for targeted sequencing?
Yes. For general information about Targeted sequencing, please visit the targeted sequencing page, the PacBio Compatible partner targeted workflows page, or contact our technical support or field application specialists if…
June 24, 2022
Quick Reference Card — Loading and Pre-Extension Recommendations for the Sequel II and IIe Systems
May 19, 2022
Quick reference card — Preparing SMRTbell sequencing libraries using SMRTbell prep kit 3.0
May 19, 2022
Procedure & checklist — Amplification of bacterial full-length 16S rRNA gene with barcoded primers
April 22, 2022