April 17, 2024
Technical overview – Whole genome and metagenome library preparation using SMRTbell prep kit 3.0
- Tutorial
- Sequel II system and Sequel IIe system, Revio system
- Library preparation, Sample preparation, Binding, Sequencing, Analysis
This technical training presentation describes workflow details for constructing whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using SMRTbell prep kit 3.0 (SPK 3.0) for sequencing on PacBio Sequel II, Sequel IIe and Revio systems. Options for multiplexing WGS samples using indexed SMRTbell adapters is discussed. Sequencing preparation workflow details, example sequencing performance data, and data analysis recommendations for de novo assembly, variant detection and shotgun metagenomics applications are also presented.