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Template preparation reagents for preparing and multiplexing SMRTbell library templates for long-read sequencing on the PacBio Sequel System.

Template preparation consumables

Quickly create libraries for Single Molecule, Real Time (SMRT) Sequencing with our template preparation kits. DNA is treated to ensure the removal of any DNA damage, and ends are repaired and circularized with the addition of SMRTbell adapters. The result is a circularized template, called a SMRTbell library.

SMRTbell templates provide a segment of single-stranded DNA to which a sequencing primer is annealed. A polymerase is bound to a primer-annealed template and the complex is ready to be loaded onto SMRT Cells.

SMRTbell Express Template Prep KitPN: 101-357-000

Quickly prepare long-insert SMRTbell libraries for the Sequel System.

Product Details

SMRTbell Template Prep Kit 1.0-SPv3PN: 100-991-900

Prepare primer-annealed, symmetric SMRTbell libraries for insert sizes ranging from 500 bp to over 20 kb.

Product Details

AMPure PBPN: 100-265-900

Magnetic beads specifically formulated for the removal of 1) small template fragments during the template preparation or 2) excess polymerase and primer from polymerase-bound SMRTbell complexes.

Product Details

Multiplexing consumables

Sequence multiple samples on the same SMRT Cell to increase throughput and enhance the efficiency of your research. Multiplexing is supported with three barcoding options providing flexibility to incorporate unique sample identifiers during target amplification or library preparation.

Illustrations of barcoded primers, barcoded universal primers, and barcoded adaptors.

  • Add barcoded primers to amplicons during (1) PCR through either target-specific primers with incorporated barcodes or (2) a two-step PCR using barcoded universal primers.
  • Add barcodes to samples with (3) ligation of barcoded SMRTbell adapters to template.

Learn more about Multiplexing, including information on barcode sequences compatible with SMRT Sequencing.

Barcoded Adapter Kit -8A/BPN: 101-081-300/400

Two sets of 8 barcoded adapters for multiplexing SMRTbell libraries in applications such as multiplexing for microbial whole genome sequencing.

Product Details

Barcoded Universal F/R Primers Plate -96PN: 100-466-100

A set of 96 barcoded primer pairs for multiplexing amplicons containing universal sequence tags.

Product Details

SMRTbell Barcoded Adapter Complete Prep -96PN: 100-514-900

A set of 96 barcoded SMRTbell adapters for multiplexing amplicons through ligation to generate SMRTbell libraries and do not require modifications to existing, pre-designed PCR primers.

Product Details

SMRTbell Damage Repair Kit -SPv3PN: 100-992-200

Perform DNA damage repair, Exonuclease III and VII treatments, and primer annealing of SMRTbell libraries prepared with SMRTbell barcoded adapters.

Product Details

Find information on Sample and Preparation Kits for PacBio RS II

 

For more information about how our Sequel System consumables can accelerate your research, contact us.