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ドラフトゲノムのその先

次世代シーケンサーにより微生物ドラフトゲノムの迅速なアセンブリができるようになりました。今、SMRTシークエンスを使えば、さらに高品質な完全ゲノム配列を決定することで微生物ゲノムと集団の真の特徴づけと理解が可能です。

信頼のある微生物完全ゲノム配列による特徴づけ

SMRTシークエンスを使えば、約50xカバレッジのアセンブリで多くの微生物におけるゲノムおよびプラズミドのゲノムをクローズでき、最短1日で実験から解析を行うことができます。

  • 最高標準のクローズした標準リファレンスゲノムを産出
  • SMRT Cellあたり最大16サンプルの微生物ゲノムをマルチプレックスすることで、標準ゲノムをコスト効率よくアセンブリ
  • トランスポゾン、フェージの挿入、その他毒性の進化における構造変異の役割を明確化
  • プラズミドを解析し、薬物耐性および伝搬経路を同定

ワークフロー:1回の実験でDNAから微生物ゲノムの特徴づけ

 

ライブラリー調製

 

 

SequelシステムでSMRTシークエンス

 

 

 

SMRT AnalysisPacBio DevNetおよび解析パートナーによるデータ解析

 

スポットライト:菌類ゲノムの理解を深めるギャップのないアセンブリ

この論文では、エフェクターと共制御遺伝子の理解を深める完全ゲノム配列決定の重要性と、菌類適応進化におけるセントロメアとテロメアの役割について報告がありました。

Thomma, B.P.H.J. et al., 2016. Mind the gap; seven reasons to close fragmented genome assemblies. Fungal Genetics and Biology, 90, pp.24–30.

スポットライト:SMRTシークエンスデGCリッチな微生物ゲノムを解明

SMRTシークエンスを使いBurkholderia pseudomallei ゲノムを解明。ロングリードのアセンブリ結果を、ショートリードおよびハイブリッドアセンブリの結果と比較。PacBioのロングリードが最も高いクオリティのアセンブリをコスト効率よく産出し、複雑な微生物ゲノムに完全な洞察をもたらしたと報告がありました。

Teng, J.L. et al., 2017. PacBio but not Illumina technology can achieve fast, accurate and complete closure of the high GC, complex Burkholderia pseudomallei two-chromosome genome. Frontiers in Microbiology, 8, p.1448.

微生物、感染症研究におけるPacBioの全ゲノムシークエンスソリューションについての詳細はぜひお問い合わせください。

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