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Workflow

The Sequel IIe Systems offer a simple workflow with rapid time to result.

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The Complete PacBio Workflow

We have introduced novel technologies at every stage of the sequencing process — from preparation to data analysis — to ensure an efficient workflow that delivers exceptional, precise results.

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Sample prep

  • High molecular weight DNA extraction for HiFi sequencing
  • Validated protocols for extraction of a diverse set of sample types

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Library preparation

  • Accepts a variety of sample types and insert sizes
  • Size-selection options to enrich for longest inserts
  • Multiplexing and barcoding solutions available

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HiFi Sequencing

  • Flexible sequencing times
  • Process up to 8 SMRT Cells 8M in one run
  • Data available for analysis as each SMRT Cell completes
  • Fully automated: No manual touch points during a sequencing run

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Analytical software

  • Variety of analysis methods available through SMRT Analysis and PacBio bioconda
  • Open source software
  • Advanced data visualization and mining

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Workflow

EMPOWER YOUR SINGLE-CELL TRANSCRIPTOME STUDY WITH MAS-SEQ

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Workflow

ADVANTAGES OF HIGHLY ACCURATE HIFI READS FOR VERIFYING AAV CONSTRUCTS

PacBio’s AAV end-to-end workflow and flexible protocol allow scientists to comprehensively profile packaged genomes as individual intact molecules to identify potential contaminants that can affect the safety, efficacy, and cost-effectiveness of AAV vector gene therapy.

  • Discover novel potential capsid constructs
  • Conduct vector quality control experiments
  • Assess the safety of resulting constructs

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Whole genome sequencing

For humans, plants, animals and microbes including de novo sequencing and variant detection

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Metagenome sequencing

Reveal the diversity of microbial communities.

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RNA sequencing

In-depth analysis of cDNA sequences across the entire transcriptome or targeted genes

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Epigenetics

Detect DNA modifications in your samples while you sequence on the PacBio platform

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Targeted sequencing

Study relevant genome targets across any regions of interest

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