X

Quality Statement

Pacific Biosciences is committed to providing high-quality products that meet customer expectations and comply with regulations. We will achieve these goals by adhering to and maintaining an effective quality-management system designed to ensure product quality, performance, and safety.

X

Image Use Agreement

By downloading, copying, or making any use of the images located on this website (“Site”) you acknowledge that you have read and understand, and agree to, the terms of this Image Usage Agreement, as well as the terms provided on the Legal Notices webpage, which together govern your use of the images as provided below. If you do not agree to such terms, do not download, copy or use the images in any way, unless you have written permission signed by an authorized Pacific Biosciences representative.

Subject to the terms of this Agreement and the terms provided on the Legal Notices webpage (to the extent they do not conflict with the terms of this Agreement), you may use the images on the Site solely for (a) editorial use by press and/or industry analysts, (b) in connection with a normal, peer-reviewed, scientific publication, book or presentation, or the like. You may not alter or modify any image, in whole or in part, for any reason. You may not use any image in a manner that misrepresents the associated Pacific Biosciences product, service or technology or any associated characteristics, data, or properties thereof. You also may not use any image in a manner that denotes some representation or warranty (express, implied or statutory) from Pacific Biosciences of the product, service or technology. The rights granted by this Agreement are personal to you and are not transferable by you to another party.

You, and not Pacific Biosciences, are responsible for your use of the images. You acknowledge and agree that any misuse of the images or breach of this Agreement will cause Pacific Biosciences irreparable harm. Pacific Biosciences is either an owner or licensee of the image, and not an agent for the owner. You agree to give Pacific Biosciences a credit line as follows: "Courtesy of Pacific Biosciences of California, Inc., Menlo Park, CA, USA" and also include any other credits or acknowledgments noted by Pacific Biosciences. You must include any copyright notice originally included with the images on all copies.

IMAGES ARE PROVIDED BY Pacific Biosciences ON AN "AS-IS" BASIS. Pacific Biosciences DISCLAIMS ALL REPRESENTATIONS AND WARRANTIES, EXPRESS, IMPLIED OR STATUTORY, INCLUDING, BUT NOT LIMITED TO, NON-INFRINGEMENT, OWNERSHIP, MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL Pacific Biosciences BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, PUNITIVE, OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER WITH RESPECT TO THE IMAGES.

You agree that Pacific Biosciences may terminate your access to and use of the images located on the PacificBiosciences.com website at any time and without prior notice, if it considers you to have violated any of the terms of this Image Use Agreement. You agree to indemnify, defend and hold harmless Pacific Biosciences, its officers, directors, employees, agents, licensors, suppliers and any third party information providers to the Site from and against all losses, expenses, damages and costs, including reasonable attorneys' fees, resulting from any violation by you of the terms of this Image Use Agreement or Pacific Biosciences' termination of your access to or use of the Site. Termination will not affect Pacific Biosciences' rights or your obligations which accrued before the termination.

I have read and understand, and agree to, the Image Usage Agreement.

I disagree and would like to return to the Pacific Biosciences home page.

Pacific Biosciences
联系方式

Sequel测序的样本和模板制备试剂盒

 

 

SMRTbell Express Template Prep Kit

101-357-000

SMRTbell Express Template Prep Kit包含完成16个建库反应的足量试剂。支持从切割后基因组DNA到SMRTbell文库的约3小时完成的实验流程。该方案仅为diffusion loading而设计。该试剂盒首先验证了构建经过片段选择的,>15kb片段文库的标准流程。重要的是,该流程并没有为扩增子测序,IsoSeq文库和未经过片段选择或平均插入片段<15kb的短片段文库而进行优化。

以下是可行实验操作步骤的一个例子。若需要完整信息,请浏览文档链接并搜索适用于您的应用的实验操作步骤。

步骤和检查列表:Preparing Greater Than 30 kb Libraries Using SMRTbell Express Template Preparation Kit
步骤和检查列表:Preparing Greater Than 15 kb Libraries Using SMRTbell Express Template Preparation Kit

SMRTbell Template Prep Kit 1.0 – SPv3

100-991-900

SMRTbell Template Prep Kit 1.0‐SPv3包含试剂支持完成插入片段从500bp到超过20kb的引物退火的SMRTBell文库的制备。 试剂量支持10个文库的构建需要。该试剂盒既包含PacBio RS II平台需要的Sequencing Primer v2,有包括Sequel系统所使用的Sequencing Primer v3。每种引物均支持10个引物退火反应。

关于大型包装,请联系您当地的技术支持和销售代表。

以下是可行实验操作步骤的一个例子。若需要完整信息,请浏览文档链接并搜索适用于您的应用的实验操作步骤。

步骤和检查列表:20 kb Template Preparation Using BluePippin Size-Selection System for Sequel Systems
步骤和检查列表:Preparing Greater than 30 kb SMRTbell Libraries Using Megaruptor Shearing

SMRTbell Damage Repair Kit – SPv3

100-992-200
SMRTbell Damage Repair Kit‐SPv3包含完成DNA修复,外切酶III和VII处理,和片段范围500bp-超过20kb的SMRTbell文库的引物退火所需的试剂。试剂量支持10个文库制备反应。与SMRTbell Barcoded Adapter Complete Prep Kit 配合使用产生用于聚合酶连接的已引物退火的 SMRTbell 文库,或单独使用,用于样本额外的DNA破碎修复。该试剂盒包含用于PacBio RSII平台的 Sequencing Primer v2,和用于Sequel系统的 Sequencing Primer v3。每个引物支持10次引物退火反应。

SMRTbell Barcoded Adapter Complete Prep Kit – 96

100-514-900
SMRTbell Barcoded Adapter Complete Prep Kit ー 96 既包含SMRTbell Barcoded Adapter Prep Kit,又包括Barcoded Adapter Plate ‐ 96。试剂量支持96个完整Barcode接头的末端修复和连接反应。配合SMRTbell Damage Repair Kit 使用完成模板制备和引物退火SMRTbell文库的产出。

SMRTbell Barcoded Adapter Prep Kit

100-465-800
SMRTbell Barcoded Adapter Prep Kit提供用于模板末端修复和SMRTbell barcode接头连接的试剂。试剂量支持96个反应。

Barcoded Adapter Plate – 96

100-466-000

Barcoded Adapter Plate – 96包括96个独特的barcode接头。试剂量支持文库制备中96个barcode接头的每个单次使用。

Barcoded Universal F/R Primer Plate – 96

100-466-100
是一块包含有barcoded引物的96孔板。板上的每个孔包含一对引物,正向引物和反向引物均含有相同的barcode。每个barcode仅存在一个反应孔中,所以板上具有96个不同的barcode。每个正向引物和反向引物均在3’端含有一段通用序列。试剂量支持文库构建中96对引物的每对单次使用。

Barcoded Adapter Kit- 8A

101-081-300

Barcoded Adapter Kit- 8A包括8个平末端Barcoded Adapter和Sequencing Primer v4。每一管Barcoded Adapter包含足够6个连接反应的试剂。此试剂盒里包含的条形码是:bc1001, bc1002, bc1004, bc1008, bc1009, bc1010, bc1012, bc1014。条形码兼容PacBio RS II和Sequel系统。Sequencing Primer v4是为扩散式上样设计的并且只在Sequel系统上测试过。

Barcoded Adapter Kit- 8B

101-081-400

Barcoded Adapter Kit- 8B包括8个平末端Barcoded Adapter和Sequencing Primer v4。每一管Barcoded Adapter包含足够6个连接反应的试剂。此试剂盒里包含的条形码是:bc1015, bc1016, bc1017, bc1018, bc1019, bc1020, bc1021, bc1022。条形码兼容PacBio RS II和Sequel系统。Sequencing Primer v4是为扩散式上样设计的并且只在Sequel系统上测试过。

AMPure PB Beads

100-265-900
AMPure PB磁珠是为 Pacific Biosciences 的SMRT 测序流程所特别配制的。
磁珠用于在样本提取和流程各个阶段中,DNA的清洁与纯化工作。试剂提供5mL体积的量。

Insert: Ampure PB
SDS: AMPure PB

以下是可行实验操作步骤的一个例子。若需要完整信息,请浏览文档链接并搜索适用于您的应用的实验操作步骤。

步骤和检查列表:500bp Template Preparation and Sequencing
步骤和检查列表:20 kb Template Preparation Using BluePippin Size-Selection

 

若有意了解更多如何运用Sequel系统的消耗品来支持您的研究,请联系我们