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Sequel测序的结合和纯化试剂盒

DNA Polymerase Binding Kit包含为PacBio系统测序制备SMRTbell文库所需的测序聚合酶和试剂。


 

Sequel Binding and Internal Ctrl Kit 2.1

101-429-300
Sequel Binding and Internal Ctrl Kit 2.1是Sequel Binding Kit 2.1和Sequel DNA Internal Control Complex 2.1的套装试剂盒。产物是DNA聚合酶和模板的混合物。Sequel Polymerase 2.1应只和Sequel Sequencing Kit 2.1一起使用。试剂分量适用于24个结合反应。Sequel DNA Internal Control Complex 2.1包含一个2kb长的固定模板,结合在Sequel DNA Polymerase 2.1上作为Sequel系统上的内部测序对照。试剂分量足够至少24个样品的掺入对照。

Sequel Binding and Internal Ctrl Kit 2.0

101-429-300
Sequel Binding and Internal Ctrl Kit 2.0是Sequel Binding Kit 2.0和Sequel DNA Internal Control Complex 2.0的套装试剂盒。Sequel Binding Kit 2.0包含了在Sequel系统测序准备过程中结合DNA模板文库和Sequel Polymerase 2.0所需的试剂。产物是DNA聚合酶和模板的混合物。Sequel Polymerase 2.0应只和Sequel Sequencing Kit 2.0一起使用。试剂分量适用于24个结合反应。Sequel DNA Internal Control Complex 2.0包含一个2kb长的固定模板,结合在Sequel DNA Polymerase 2.0上作为Sequel系统上的内部测序对照。试剂分量足够至少24个样品的掺入对照。

MagBead Kit v2

100-676-500
MagBead Kit v2包含的试剂支持捕获DNA聚合酶和被测模板复合物,装载入PacBio系统的测序Cell上。试剂盒的组分有:MagBeads, MagBead Binding Buffer Kit v2和Wash Buffer Kit v2。
Procedure & Checklist: Preparing MagBeads for Sequencing

MagBead

100-125-900
MagBeads含有2.88 mL的MagBeads,用于捕获DNA聚合酶和被测模板复合物,装载入PacBio系统的测序Cell上。MagBeads通常情况下与MagBead Binding和Wash Buffer Kit配合使用。

MagBead Wash Buffer Kit v2

101-046-500
MagBead Wash Buffer Kit v2通常情况下与 MagBead Binding Buffer Kit 和 MagBeads 配合使用,利用 Pacific Biosciences Binding 计算工具来完成连接DNA/聚合酶复合物的实验步骤。

MagBead Binding Buffer Kit v2

101-046-400
MagBead Binding Buffer Kit v2通常情况下与MagBead Binding Buffer Kit和MagBeads 配合使用,利用Pacific Biosciences Binding计算工具来完成连接DNA/聚合酶复合物的实验步骤。

SMRTbell Clean Up Column v2 Kit-Dif

101-184-100
SMRTbell Clean Up Column v2 Kit-Dif包含20个纯化柱和用于柱子起始状态下的缓冲液,该缓冲液兼容DNA/聚合酶复合物DIFFUSION loading所使用的缓冲液。SMRTbell CleanUp Column v2可以去除DNA与聚合酶连接反应中过量的聚合酶和引物。SMRTbell CleanUp Column Kit v2‐Dif包含各一个以下组分:SMRTbell Clean Up Columns v2 (PN: 101‐175‐400)和MagBead Binding Buffer Kit v2 (PN: 101‐046‐400)。

SMRTbell Clean Up Column v2 Kit-Mag

101-303-600
SMRTbell Clean Up Column v2 Kit-Mag包含20个纯化柱和用于柱子起始状态下的缓冲液,该缓冲液兼容DNA/聚合酶复合物 MAGBEAD loading所使用的缓冲液。SMRTbell CleanUp Column v2可以去除DNA与聚合酶连接反应中过量的聚合酶和引物。SMRTbell CleanUp Column Kit v2‐Mag 包含各一个以下组分:SMRTbell Clean Up Columns v2 (PN: 101‐175‐400) 和MagBead Wash Buffer Kit v2 (PN: 101‐046‐500)。

Loading Cleanup Binding Kit (10 rxn)

100-715-300
Loading Cleanup Bead Kit (10 rxn)包含聚合酶连接反应后过量聚合酶去除的试剂。Loading Cleanup Beads仅用于样本文库以diffusion的方式加载到Sequel SMRT Cell 1M上。试剂量支持10个聚合酶连接产物的纯化反应。
Not applicable

若有意了解更多如何运用Sequel系统的消耗品来支持您的研究,请联系我们