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Sequel测序的消耗品

Sequel系统有四种主要类型的消耗品,如下图所示。

 

SMRT-Sequencing-workflow_v2

 

四种主要类型的消耗品如下:

1. SMRTbell样本和模板制备试剂盒

  • 将DNA(基因组DNA或扩增子)和cDNA样本转化成引物退火结合的SMRTbell文库形式
  • 可在实验台上手动操作,或利用自动化的液体处理系统

2. DNA聚合酶结合试剂盒

  • 引物退火结合的SMRTbell模板与DNA聚合酶结合,为测序做准备
  • 与聚合酶结合的SMRTbell模板与磁珠退火结合,以便上样至PacBio系统

3. 测序消耗品

  • 在仪器上测序所必需的SMRT Cell和DNA测序试剂

4. 耗材

  • 专为Sequel系统而设计和优化的多孔板、隔板和吸头

除了主要的流程步骤,其它的上游流程适用于:

1. 基于探针杂交的靶向富集

  • 在模板制备反应前开展靶向富集的操作方案

2. 多重制备PCR扩增子

  • 预设计的条形码和专门的模板制备试剂盒用于样本和模板制备试剂盒中的添加条形码流程

SMRT测序项目计算器

将您的测序项目告知我们,并估算利用Sequel系统开展以下项目所需的SMRT Cell和运行时间:

若有意了解更多如何运用Sequel系统的消耗品来支持您的研究,请联系我们