X

Quality Statement

Pacific Biosciences is committed to providing high-quality products that meet customer expectations and comply with regulations. We will achieve these goals by adhering to and maintaining an effective quality-management system designed to ensure product quality, performance, and safety.

X

Image Use Agreement

By downloading, copying, or making any use of the images located on this website (“Site”) you acknowledge that you have read and understand, and agree to, the terms of this Image Usage Agreement, as well as the terms provided on the Legal Notices webpage, which together govern your use of the images as provided below. If you do not agree to such terms, do not download, copy or use the images in any way, unless you have written permission signed by an authorized Pacific Biosciences representative.

Subject to the terms of this Agreement and the terms provided on the Legal Notices webpage (to the extent they do not conflict with the terms of this Agreement), you may use the images on the Site solely for (a) editorial use by press and/or industry analysts, (b) in connection with a normal, peer-reviewed, scientific publication, book or presentation, or the like. You may not alter or modify any image, in whole or in part, for any reason. You may not use any image in a manner that misrepresents the associated Pacific Biosciences product, service or technology or any associated characteristics, data, or properties thereof. You also may not use any image in a manner that denotes some representation or warranty (express, implied or statutory) from Pacific Biosciences of the product, service or technology. The rights granted by this Agreement are personal to you and are not transferable by you to another party.

You, and not Pacific Biosciences, are responsible for your use of the images. You acknowledge and agree that any misuse of the images or breach of this Agreement will cause Pacific Biosciences irreparable harm. Pacific Biosciences is either an owner or licensee of the image, and not an agent for the owner. You agree to give Pacific Biosciences a credit line as follows: "Courtesy of Pacific Biosciences of California, Inc., Menlo Park, CA, USA" and also include any other credits or acknowledgments noted by Pacific Biosciences. You must include any copyright notice originally included with the images on all copies.

IMAGES ARE PROVIDED BY Pacific Biosciences ON AN "AS-IS" BASIS. Pacific Biosciences DISCLAIMS ALL REPRESENTATIONS AND WARRANTIES, EXPRESS, IMPLIED OR STATUTORY, INCLUDING, BUT NOT LIMITED TO, NON-INFRINGEMENT, OWNERSHIP, MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL Pacific Biosciences BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, PUNITIVE, OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER WITH RESPECT TO THE IMAGES.

You agree that Pacific Biosciences may terminate your access to and use of the images located on the PacificBiosciences.com website at any time and without prior notice, if it considers you to have violated any of the terms of this Image Use Agreement. You agree to indemnify, defend and hold harmless Pacific Biosciences, its officers, directors, employees, agents, licensors, suppliers and any third party information providers to the Site from and against all losses, expenses, damages and costs, including reasonable attorneys' fees, resulting from any violation by you of the terms of this Image Use Agreement or Pacific Biosciences' termination of your access to or use of the Site. Termination will not affect Pacific Biosciences' rights or your obligations which accrued before the termination.

I have read and understand, and agree to, the Image Usage Agreement.

I disagree and would like to return to the Pacific Biosciences home page.

Pacific Biosciences
联系方式

体验专为PacBio数据而优化的从头组装先进算法

这些算法带来了最高的重叠群N50,最少的重叠群,单核苷酸多态性和结构变异检测,超过99%一致性序列准确性。其它工具也能够对新组装、重叠群和可视化进行广泛的质量评估。

SMRTAnalysis的从头组装算法

HGAP

Hierarchical Genome Assembly Process(HGAP)利用单一文库类型生成了全面、高度准确的从头组装。HGAP包括预组装,利用Celera Assembler进行de novo组装,以及利用Quiver进行组装修正。

BridgeMapper

BridgeMapper通过对PacBio的数据进行拆分比对来评估基因组组装的质量,并在SMRT View中显示。

SMRTAnalysis的从头组装应用程序

RS_HGAP Assembly.3

此应用程序利用单一类型的文库开展高质量的从头组装。它针对速度进行了优化,比RS_HGAP_Assembly.2更快。HGAP Assembly.3包括预组装,利用PacBio AssembleUnitig 的de novo组装,以及利用Quiver进行组装修正,在微生物组装上的速度明显提升。PacBio的AssembleUnitig模块取代了Celera Assembler中最耗时的一步。

RS_HGAP Assembly.2

此应用程序利用单一文库类型开展从头组装,并针对质量进行了优化。HGAP_Assembly.2包括预组装、利用Celera Assembler进行从头组装,并利用Quiver进行组装修正。

RS_Bridgemapper

BridgeMapper对PacBio数据进行拆分比对(拆分读取序列映射)。拆分比对显示在SMRT View中,具体显示为部分映射到不同位置的序列。 如果部分序列映射到参考序列的不连续部分,则表明参考序列与真实基因组之间存在结构差异。 这将帮助您了解在传统映射过程中无法比对的那部分数据的性质和起源。 它也可以用于重测序应用中的基因融合检测。

RS_PreAssembler

此应用程序构建了一套高度准确的长读取序列,用于从头组装。它收集每条超过最短长度的读取序列,将所有序列与之比对,修正边缘,然后获得一致性序列。它使用Hierarchical Genome Assembly Process(HGAP)。

更多资源

  1. SMRT Portal中的HGAP
  2. HGAP Whitelisting教程
  3. 评估HGAP组装结果
  4. BridgeMapper

 

若有意了解如何运用这种创新的软件来支持您的研究工作,请联系我们