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超越基因组草图

新一代测序技术让微生物基因组草图的快速组装成为现实。不过,现在有了SMRT测序,我们能够大大超越基因组草图,获得最高质量的完整基因组,并真正鉴定和了解微生物基因组和群落。

轻松自信地完成微生物全基因组测序

对于大多数微生物而言,包括附属质粒的封闭基因组可以用50倍覆盖度的单分子实时(SMRT)测序数据进行组装,所需时间可少至1天。

  • 生成白金标准的封闭参考基因组
  • 通过在一个SMRT Cell上多达微生物16重化,经济地组装金标准基因组
  • 阐明转座子,噬菌体插入和其它结构变异在毒力进化中的角色
  • 复原质粒以跟踪抗药性和传递通路

工作流程: 在单个实验中从DNA得到表征过的微生物基因组

 

文库制备

 

 

Sequel系统进行SMRT 测序

 

 

 

通过SMRT Analysis, PacBio DevNet和我们的 分析合作伙伴进行数据分析

 

研究聚焦:无缺口组装让您更好地了解真菌基因组

最近一项研究强调了完整基因组在了解效应物和共调控基因中的重要性,以及着丝粒和端粒在真菌物种的适应性基因组进化中的作用。深入了解这项研究:

Thomma, B.P.H.J. et al., 2016. Mind the gap; seven reasons to close fragmented genome assemblies. Fungal Genetics and Biology, 90, pp.24–30.

研究聚焦:利用SMRT测序来解析富含GC的微生物基因组

科学家利用SMRT测序来解析类鼻疽伯克氏菌(Burkholderia pseudomallei)的基因组,并比较了混合组装和短读长组装的结果。他们的结论是,PacBio长读长测序实现最高质量且经济高效的组装,获得无缺口的复杂微生物基因组。深入了解这项研究:

Teng, J.L. et al., 2017. PacBio but not Illumina technology can achieve fast, accurate and complete closure of the high GC, complex Burkholderia pseudomallei two-chromosome genome. Frontiers in Microbiology, 8, p.1448.

若有意了解PacBio适用于微生物学和传染病研究的真正全基因组测序,请联系我们

Selected Resources