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以高分辨率捕获感兴趣的区域

在农业研究中,进化基因组特征的发现和动植物的改良都依赖与表型差异相关的SNP的鉴定。为了鉴定负责特定表型的基因,含有相关SNP的区域必须精细定位,这通常依靠靶向测序。不过,这些区域通常很大,大约为几千个碱基,还可能包含现有技术难以鉴定的结构变异。

灵活靶定您感兴趣的所有区域

单分子实时(SMRT)测序为靶定感兴趣的区域提供了一个灵活的解决方案,而无论其大小如何,可提供相关基因的最完整视图。它的读长和准确性有助于改善精细定位,并简化大型区域的组装。

SMRT测序让您能够:

  • 对大的扩增子进行高效的多重分析,以便快速筛查和鉴定变异
  • 发现单体型特异性标记,以改善多倍体的育种
  • 准确重新组装Mb大小的整个区域,用于基因连锁研究
  • 确认转基因的插入位点,并验证基因编辑事件
  • 研究富含信息的区域,以确定进化或驯养历史
  • 捕获不同大小的完整基因,为基因研究带来灵活高效的实验设计

工作流程:从靶向区域到单碱基水平的分辨

 

 

 

 

利用PacBio系统进行SMRT测序

  • 利用Sequel系统的优势降低项目成本,产生更多读取序列
  • 调整运行时间(0.5到20小时),以便最大限度提高样本通量和周转时间
  • 通过避免比对和系统错误,获得高于 99.999%的一致性序列准确性
  • 多次滚动测序单个环化模板可达到高度准确的单分子一致性序列,以解析复杂群体
 

利用SMRT AnalysisPacBio DevNet进行数据分析

研究聚焦:靶向测序揭示牛TLR4受体的多样性

科学家利用PacBio测序来评估两个古老品种的牛中Toll样受体基因TLR4的等位基因组成。他们鉴定出8个单核苷酸多态性,可定相为18个单体型,这代表了很大一部分的已知多态性。深入了解这项研究:

Novák, K. et al., 2017. Diversity of the TLR4 immunity receptor in Czech native cattle breeds revealed using the Pacific Biosciences sequencing platform. Animal Biotechnology, 28(3), pp.228–236.

若有意了解SMRT测序如何全面查看您的靶向区域,请联系我们

Selected Resources