X

Quality Statement

Pacific Biosciences is committed to providing high-quality products that meet customer expectations and comply with regulations. We will achieve these goals by adhering to and maintaining an effective quality-management system designed to ensure product quality, performance, and safety.

X

Image Use Agreement

By downloading, copying, or making any use of the images located on this website (“Site”) you acknowledge that you have read and understand, and agree to, the terms of this Image Usage Agreement, as well as the terms provided on the Legal Notices webpage, which together govern your use of the images as provided below. If you do not agree to such terms, do not download, copy or use the images in any way, unless you have written permission signed by an authorized Pacific Biosciences representative.

Subject to the terms of this Agreement and the terms provided on the Legal Notices webpage (to the extent they do not conflict with the terms of this Agreement), you may use the images on the Site solely for (a) editorial use by press and/or industry analysts, (b) in connection with a normal, peer-reviewed, scientific publication, book or presentation, or the like. You may not alter or modify any image, in whole or in part, for any reason. You may not use any image in a manner that misrepresents the associated Pacific Biosciences product, service or technology or any associated characteristics, data, or properties thereof. You also may not use any image in a manner that denotes some representation or warranty (express, implied or statutory) from Pacific Biosciences of the product, service or technology. The rights granted by this Agreement are personal to you and are not transferable by you to another party.

You, and not Pacific Biosciences, are responsible for your use of the images. You acknowledge and agree that any misuse of the images or breach of this Agreement will cause Pacific Biosciences irreparable harm. Pacific Biosciences is either an owner or licensee of the image, and not an agent for the owner. You agree to give Pacific Biosciences a credit line as follows: "Courtesy of Pacific Biosciences of California, Inc., Menlo Park, CA, USA" and also include any other credits or acknowledgments noted by Pacific Biosciences. You must include any copyright notice originally included with the images on all copies.

IMAGES ARE PROVIDED BY Pacific Biosciences ON AN "AS-IS" BASIS. Pacific Biosciences DISCLAIMS ALL REPRESENTATIONS AND WARRANTIES, EXPRESS, IMPLIED OR STATUTORY, INCLUDING, BUT NOT LIMITED TO, NON-INFRINGEMENT, OWNERSHIP, MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL Pacific Biosciences BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, PUNITIVE, OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER WITH RESPECT TO THE IMAGES.

You agree that Pacific Biosciences may terminate your access to and use of the images located on the PacificBiosciences.com website at any time and without prior notice, if it considers you to have violated any of the terms of this Image Use Agreement. You agree to indemnify, defend and hold harmless Pacific Biosciences, its officers, directors, employees, agents, licensors, suppliers and any third party information providers to the Site from and against all losses, expenses, damages and costs, including reasonable attorneys' fees, resulting from any violation by you of the terms of this Image Use Agreement or Pacific Biosciences' termination of your access to or use of the Site. Termination will not affect Pacific Biosciences' rights or your obligations which accrued before the termination.

I have read and understand, and agree to, the Image Usage Agreement.

I disagree and would like to return to the Pacific Biosciences home page.

Pacific Biosciences
联系方式

解析复杂度

微生物群落的更深入探索需要不断改良的方法。长读长测序能够提供物种水平的分辨率和全面的表征,从全长16S rRNA到鸟枪法宏基因组学,实现完整的基因组组装,从而推动功能研究。

探索形式和功能

利用PacBio系统开展的单分子实时(SMRT)测序可实现长的测序读长、高准确性和单分子分辨率,让您能够:

  • 利用长读长宏基因组分析鉴定全长基因,而无需组装
  • 在群落组成和系统发育上实现菌株水平的更高分辨率
  • 在全基因组鸟枪法宏基因组组装中重建更长的重叠群,并发现新的基因和基因簇
  • 利用表观基因组数据将来自近缘菌株的重叠群和质粒相关联

工作流程:从DNA到清晰分辨的微生物群落

文库制备

 

 

利用Sequel系统开展SMRT测序

  • 可实现高达 ~15 kb的平均读长,其中某些序列读长可长达100 kb
  • 每个SMRT Cell可提供超过25万条达到QV30的序列以分辨16S rDNA序列
  • 宏基因组的从头组装:推荐每个基因组50倍覆盖度
 

 

利用SMRT AnalysisPacBio DevNet服务伙伴开展数据分析

研究聚焦:准确绘制真菌区系,了解人类健康

科学家研究了各种测序技术,希望建立最严格的方法来绘制健康个体的肠道真菌区系。结果表明,PacBio SMRT测序最准确地代表了宏基因组群体图谱。深入了解这项研究:

Motooka, D. et al., 2017. Fungal ITS1 deep-sequencing strategies to reconstruct the composition of a 26-species community and evaluation of the gut mycobiota of healthy Japanese individuals. Frontiers in Microbiology, 8, p.238.

若有意了解如何全面解析您的微生物群落,请联系我们

Selected Resources