To bring personalized medicine to all patients, cancer researchers need more reliable and comprehensive views of somatic variants of all sizes that drive cancer biology.
The Sequel II System, powered by Single Molecule, Real Time (SMRT) Technology, delivers highly accurate long reads for a comprehensive view of genomes, transcriptomes and epigenomes.
Learn how Single Molecule, Real-Time (SMRT) Sequencing and the Sequel IIe System and will accelerate your research by delivering highly accurate long reads to provide the most comprehensive view of genomes, transcriptomes and epigenomes.
Korean service provider DNA Link has established strong expertise with the PacBio sequencing platform in response to high global demand for the technology.
The Sequel System, powered by Single Molecule, Real Time (SMRT) Technology, delivers long reads, high consensus accuracy, uniform coverage and epigenetic characterization.
Jonas Korlach, of PacBio, discusses the use of SMRT sequencing to detect DNA modifications.
Tyson Clark, a scientist at PacBio, demonstrates the detection and identification of damaged DNA using SMRT Sequencing. With the platform’s ability to see base modifications, Clark notes that the polymerase kinetics can distinguish between different types of DNA damage as well — such as oxidative, radiation, and alkylation. This could help in studies of cancer and aging, where DNA damage is an important factor.
Paul Coupland and his team at the Wellcome Trust Sanger Institute have developed a sequencing method on the PacBio System for small DNA molecules that avoids the need for a standard library preparation. To date this approach has been applied toward sequencing single-stranded and double-stranded viral genomes, bacterial plasmids, plasmid vector models for DNA-modification analysis, and linear DNA fragments covering an entire bacterial genome. Using direct sequencing it is possible to generate sequence data from as little as 1 ng of DNA, offering a significant advantage over current protocols which typically require 400–500 ng of sheared DNA for the library…
Jonas Korlach, CSO of PacBio, discusses the revival of finished genomes the microbial community will see with long read data, emphasizing that for certain organisms such as rapidly evolving microbes, having a de novo finished genome will be more useful than creating a draft based on a previous related reference genome. Korlach describes two bioinformatic methods from PacBio, a hierarchical genome assembly process (HGAP) and an consensus caller (Quiver), which are used to generate finished genomes from just long-read PacBio data, with final genome sequence accuracies over 99.999%. Korlach demonstrates the ability of PacBio data to generate closed, high-quality de…
In this AGBT plenary talk, Jonas Korlach presented a number of collaborative studies between PacBio and other institutions to make use of highly accurate, long-read sequence data, which has led to a revival of finished genomes. Examples from the infectious disease or pathogen realm included Pertussis, Salmonella, and Listeria, all of which now have closed genomes from PacBio-generated data. Korlach also reported on epigenomic information in Salmonella and Listeria, indicating potential new forms of DNA modifications.
Sebastian Suerbaum from Hannover Medical School shows that genome-wide methylation patterns in Helicobacter pylori are highly complex and diverge significantly between strains of the microbe. He presents a full-methylome analysis of two H. pylori strains, finding 32 total methylated motifs with just seven shared between strains. Of the 32 motifs, 11 were new discoveries.
Garth Ehrlich from the Center for Genomic Sciences at Allegheny Singer Research Institute reports on new studies of pneumococcal epigenetics. Streptococcus pneumonia, which causes more than 1.6 million deaths annually, has a highly plastic genome. Methylation analysis with SMRT Sequencing found a novel modification in addition to the expected epigenetic changes.
Jonas Korlach, CSO of PacBio, discusses the scientific value of >10 kb, unbiased sequencing reads for an expanding range of applications. Single molecule, real-time (SMRT) Sequencing technology has rapidly advanced in read lengths, throughput and adoption in the past year – a review of published work from a variety of researchers utilizing these new capabilities is also conducted.