Chetana Pandya, a bioinformatician at the Icahn School of Medicine at Mount Sinai discusses how the institute is using their PacBio Systems in a variety of cancer research project including detecting somatic fusions events in patient-derived cancer samples and validating somatic variants.
Fritz Sedlazeck, a bioinformatician at John Hopkins, discusses why he's excited to use long-read sequencing from PacBio to resolve structural variants.
Robert Morey of Synthetic Genomics discusses how they are using SMRT Sequencing for process development of long, synthetic DNA constructs. PacBio Systems allows them to do larger experiments, cheaper and faster than more traditional approaches, like Sanger Sequencing.
See what PacBio users had to say about SMRT Sequencing at the Plant and Animal Genome (PAG) Conference in San Diego. This brief video captures highlights from posters, presentations, and the exhibit hall. See how SMRT Sequencing benefits plant and animal scientists in their genomic investigations. [Engligh, some Mandarin]
Chad Nusbaum from the Broad Institute talks about the sequencing projects that will now be possible with the new Sequel System from PacBio, including efforts to sequence human genomes that currently are limited by cost.
Yunfei Guo, a grad student at the University of Southern California, discusses the benefits of SMRT Sequencing: very long reads that make it possible to resolve long repetitive regions and discover structural variants, and a random error mode that allows for extremely high accuracy.
Alex Dainis from Stanford University says the Sequel System from PacBio will offer more power to multiplex samples more efficiently.
Bobby Sebra from the Icahn School of Medicine at Mount Sinai talks about how he uses PacBio sequencing for disease biology, pathogen surveillance, metagenomic sequencing, and more. With reads 70 kb or even longer, SMRT Sequencing lets him characterize tandem repeats and other structurally relevant elements that are inaccessible with short-read sequencing.
Anne Deslattes Mays from Georgetown University describes how long-read sequencing provided important biological answers after three years of short-read sequencing failed to generate enough information for her gene discovery project. She says long reads are essential for understanding alternative splicing, RNA editing, and genome rearrangements.
Dan Geraghty from the Fred Hutchinson Cancer Research Center says that PacBio sequencing “gives us access to genomic sequence data that's not available by any other means.” He's using it to understand the genetic basis of autoimmune diseases and has for the first time gotten answers to problems that have been challenging researchers for decades.
Yuta Suzuki from the University of Tokyo details his experience using SMRT Sequencing for generating data for haplotype-specific epigenetic analysis.
Commentary from PacBio users on their applications of SMRT Sequencing, including Ulf Gyllensten (Uppsala University), Tim Smith (USDA-ARS) and Bobby Sebra (Icahn School of Medicine)
PacBio customers discuss their applications of PacBio SMRT Sequencing and long reads, including Lemuel Racacho (Children's Hospital of Eastern Ontario Research Institute), Matthew Blow (JGI), Yuta Suzuki (U. of Tokyo), Daniel Geraghty (Fred Hutchinson Cancer Center), and Mike Schatz (CSHL)
Ulf Gyllensten shares how whole genome sequencing with PacBio is revealing variation and regions of the genome never previously known.
Lemuel Racacho at the Children's Hospital of Eastern Ontario says that his team chose SMRT Sequencing to detect longer isoforms of noncoding RNAs and to survey numerous transcripts. Continuous long reads offer a chance to see a lot of RNA missed by other technologies, he adds.