PacBio Sequencing and software enable the generation of highly accurate (>99.9%) long reads. HiFi reads are accurate, essential, affordable, and can be used across a range of applications, including detection of all variant types, from single nucleotides to structural variants. PacBio’s end-to-end solutions feature library preparation paired with push-button analysis to support numerous workflows so you can run projects quickly and easily.
In this talk, Aaron Wenger from PacBio uses industry examples to describe how using highly accurate long-reads, or HiFi reads, provides the most comprehensive result, giving you greater than 99.9% accuracy, up to 25 kb long.
In this talk, Jonas Korlach, PhD, Chief Scientific Officer at PacBio describes how using PacBio HiFi reads, which are greater than 99.9% accurate and up to 25 kb long, led to the detection of structural variants in examples of previously unexplained rare genetic diseases. Genetic diseases affect as much as 10% of the population and over 50% of cases currently remain unexplained. Similarly, Mendelian diseases include over 8,500 described disorders, however at present ~40% have an unknown genetic cause. In addition, he highlights the strength of complete, phased, high-accuracy human WGS for simultaneously yielding high-quality information about any other locus…
In this panel discussion, service providers share their experiences in bringing PacBio Systems to their labs, from the purchasing process, through managing demand for instrument time, and describe how PacBio solutions offer customers the most informative data available.
Learn how HiFi reads are empowering leading core labs and service providers, and how the new Sequel IIe System, which directly outputs HiFi reads, is making it easier than ever before to get started with HiFi reads or add capacity.
This animation depicts a process by which single molecule SMRTbells are loaded in the Zero Mode Waveguides (ZMWs) of the PacBio RS II sequencing system using the automated MagBead Station.
The Sequel System, powered by Single Molecule, Real-Time (SMRT) Technology, delivers long reads, high consensus accuracy, uniform coverage and epigenetic characterization. This newly introduced platform provides higher throughput, a reduced footprint and lower sequencing project costs compared to the PacBio RS, the original long-read sequencer.