Asset Tag: Ultra-low DNA Input
Application note — Considerations for using the low and ultra-low DNA input workflows for whole genome sequencing
As the foundation for scientific discoveries in genetic diversity, sequencing data must be accurate and complete. With highly accurate long-read sequencing, or HiFi sequencing, there is no longer a compromise between read length and accuracy. HiFi sequencing enables some of the highest quality de novo genome assemblies available today as well as comprehensive variant detection in human samples. PacBio HiFi libraries constructed using our standard library workflows require at least 3 µg of DNA input per 1 Gb of genome length, or ~10 µg for a human sample. For some samples it is not possible to extract this amount of DNA for sequencing. For samples where between 300 ng and 3 ug of DNA is available, the Low DNA Input Workflow enables users to generate high-quality genome assemblies of small-bodied organisms. For samples where even less DNA is available (as low as 5 ng), the amplification-based Ultra-Low DNA Input Workflow is available.
PAG Conference: PacBio update on products and HiFi applications
In this talk at PAG 2020, PacBio Plant and Animal Sciences Marketing Manager Michelle Vierra discusses recent updates to Single Molecule, Real-Time (SMRT) Sequencing technology, including the Sequel II System,…
Webinar: No Organism Too Small – Build high-quality genome assemblies of small organisms with HiFi sequencing
In this webinar you will hear how several researchers have overcome the challenges of sequencing organisms with small body size using the new low and ultra-low DNA input methods from…
Virtual Global Summit: Technical Tutorial – Choosing the best library prep for HiFi sequencing
In this PacBio Virtual Global Summit 2020 presentation, Nicole Newell of PacBio provides an overview of the library preparation kits available for highly accurate long read (HiFi sequencing) applications.