June 1, 2021  |  

The “Art” of shotgun sequencing

2015 SMRT Informatics Developers Conference Presentation Slides: Jason Chin of PacBio highlighted some of the challenges for shotgun assembly while suggesting some potential solutions to obtain diploid assemblies, including the FALCON method.


June 1, 2021  |  

MinHash for overlapping and assembly

2015 SMRT Informatics Developers Conference Presentation Slides: Sergey Koren of National Biodefense Analysis and Countermeasures Center (NBACC) provided an overview of the MHAP algorithm, a method for assembling large genomes with Sing-Molecule Sequencing and locality sensitive hashing. Using MHAP, Koren produced a human assembly (CHM1) with a contig N50 of >23 Mb.


June 1, 2021  |  

Making the most of long reads: towards efficient assemblers for reference quality, de novo reconstructions

2015 SMRT Informatics Developers Conference Presentation Slides: Gene Myers, Ph.D., Founding Director, Systems Biology Center, Max Planck Institute delivered the keynote presentation. He talked about building efficient assemblers, the importance of random error distribution in sequencing data, and resolving tricky repeats with very long reads. He also encouraged developers to release assembly modules openly, and noted that data should be straightforward to parse since sharing data interfaces is easier than sharing software interfaces.


June 1, 2021  |  

Transcriptome analysis using Hybrid-Seq

2015 SMRT Informatics Developers Conference Presentation Slides: Kin Fau Au of the University of Iowa presented on a suite of transcriptome analysis tools for junction detection, error correction, isoform detection and prediction, and gene fusion.


June 1, 2021  |  

Introduction to SMRT informatics developers conference

2015 SMRT Informatics Developers Conference Presentation Slides: Kevin Corcoran of PacBio provided a brief review of community involvement in the development of analysis tools and showed a preview of upcoming sample preparation, chemistry and informatics improvements.


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