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Friday, February 5, 2021

Webinar: Sequencing 101 – How long-read sequencing improves access to genetic information

In this webinar, Kristin Mars, Sequencing Specialist, PacBio, presents an introduction to PacBio’s technology and its applications followed by a panel discussion among sequencing experts. The panel discussion addresses such things as what long reads are and how are they useful, what differentiates PacBio long-read sequencing from other technologies, and the applications PacBio offers and how they can benefit scientific research.

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Friday, February 5, 2021

PAG Conference: PacBio update on products and HiFi applications

In this talk at PAG 2020, PacBio Plant and Animal Sciences Marketing Manager Michelle Vierra discusses recent updates to Single Molecule, Real-Time (SMRT) Sequencing technology, including the Sequel II System, updated protocols for low-input as well as other upcoming developments.

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Friday, February 5, 2021

PacBio Workshop: Understanding the biology of genomes with HiFi sequencing

The utility of new highly accurate long reads, or HiFi reads, was first demonstrated for calling all variant types in human genomes. It has since been shown that HiFi reads can be used to generate contiguous, complete, and accurate human genomes, even in repeat structures such as centromeres and telomeres. In this virtual workshop scientists from PacBio as well as Tina Graves-Lindsay from the McDonnell Genome Institute at Washington University share the many improvements we’ve made to HiFi sequencing in the past year, tools that take advantage of HiFi data for variant detection and assembly, and examples in numerous genomics…

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Friday, February 5, 2021

Webinar: A HiFi View – Sequencing the gut microbiome with highly accurate long reads

In this webinar, Dr. Ashby gives attendees a brief update on PacBio’s metagenomics solutions on the Sequel II System. Then, Dr. Ma, University of Maryland School of Medicine, discusses her work using long read sequencing to identify high-resolution microbial biomarkers associated with leaky gut syndrome in premature infants. Finally, Dr. Weinstock, The Jackson Laboratory, talks about the potential of highly accurate long reads to enable strain-level resolution of the human gut microbiome by resolving intraspecies variation in multiple copies of the 16S gene.

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Friday, February 5, 2021

Video: Introduction to PacBio highly accurate long-read sequencing

PacBio Sequencing is powered by Single Molecule, Real-Time (SMRT) Sequencing technology. The Sequel II System offers the affordable, highly accurate long reads needed to gain comprehensive views of genomes, transcriptomes, and epigenomes. Watch this video to get to know the Sequel II System, explore the key advantages of SMRT Sequencing, and learn how its applications can be used to drive new discoveries.

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Friday, February 5, 2021

Webinar: Bioinformatics lunch & learn – A quick guide to metagenomic analysis with PacBio HiFi reads

In this webinar, scientists from PacBio share how using Single Molecule, Real-Time (SMRT) Sequencing, you can generate highly accurate long reads – HiFi reads – with 99% accuracy (Q20) and read lengths of 10 kb or more. This high resolution of each single molecule enables species or strain-level profiling of complex populations in both targeted and shotgun sequencing experiments. Genome assemblies are more cost effective than ever before when sequencing metagenomics samples with the Sequel II System.

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Friday, February 5, 2021

Virtual Global Summit: Shining Light On An Elusive Bug – A comparative metagenome-assembled genome analysis of “Candidatus Lachnocurva vaginae”, formerly known as Bacterial Vaginosis Associated Bacterium – 1 (BVAB1)

In this PacBio Virtual Global Summit 2020 presentation, JoAnna Holm of the University of Maryland shares her work on Bacterial Vaginosis Associated bacterium 1 (BVAB1), an as-yet uncultured bacterial species found in the human vagina that belongs to the family Lachnospiraceae within the order Clostridiales. Using the PacBio Sequel II System the first circularized metagenome-assembled genome (cMAG) of BVAB1 of this species has been produced, allowing for proper phylogenetic placement of the species in its own genus “Candidatus Lachnocurva vaginae” and genomic analysis to better understand its pathogenicity.

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Friday, February 5, 2021

Virtual Global Summit: Using the unique capabilities of the Sequel II System for de novo genome assembly and full-length 16S sequencing

In this PacBio Virtual Global Summit 2020 presentation, Alvaro Hernandez of University of Illinois at Urbana-Champaign shares his experience introducing the Sequel II System to the DNA Sequencing facility at his institution. Hernandez also goes over recommendations to ensure high-quality de novo genome assemblies and benefits of full-length 16S sequencing, compared to the sequencing of variable regions. 

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Tuesday, April 21, 2020

Generating amplicon reads for microbial community assessment with next-generation sequencing.

Marker gene amplicon sequencing is often preferred over whole genome sequencing for microbial community characterization, due to its lower cost while still enabling assessment of uncultivable organisms. This technique involves many experimental steps, each of which can be a source of errors and bias. We present an up-to-date overview of the whole experimental pipeline, from sampling to sequencing reads, and give information allowing for informed choices at each step of both planning and execution of a microbial community assessment study. When applicable, we also suggest ways of avoiding inherent pitfalls in amplicon sequencing. © 2019 The Society for Applied Microbiology.

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