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Capture regions of interest in high resolution

Combining PacBio long-read sequencing with targeted sequence capture or bacterial artificial chromosomes (BACs) provides a powerful tool for a comprehensive view of specific genomic regions of interest, regardless of size.

Flexible workflows for all your important genomic targets

Single Molecule, Real-Time (SMRT) Sequencing offers a flexible solution for targeting regions of interest, from several hundred base pairs to 20 kb, and delivers the most comprehensive view of the associated genes. With high-fidelity, long reads (>99% single-molecule read accuracy) it also delivers the read length and accuracy needed to improve fine mapping and simplify the assembly of complex portions of the genome. SMRT Sequencing gives you the ability to:

  • Rapidly screen and identify all variants
  • Discover haplotype-specific markers
  • Resolve difficult-to-sequence regions, regardless of size
  • Confirm insertion sites of transgenes and validate gene editing events

Workflow: from sample to base-level resolution

 

Sample & Library Preparation
Obtain targeted sequences with flexible sample and library workflows

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Sequencing
Maximize output and turn-around time with adjustable run parameters on the Sequel System.

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Data Analysis
Make discoveries using bioinformatics tools in SMRT Analysis or PacBio DevNet.

Learn More


 

Application Brief: Learn more about these best practices for targeted sequencing

 

 


 

Spotlight: Codfish study employs target capture to explore evolution

Researchers used SMRT Sequencing and Roche NimbleGen target capture to resolve the copy number variation and organization of hemoglobin (Hb) genes and their flanking regions across eight species of codish. Explore this research further:

Khang Hoff, S.N., et al., 2018. Long-read sequence capture of the hemoglobin gene clusters across species. bioRxiv, Preprint.

Spotlight: Amplicon sequencing on a massive scale

Scientists used SMRT Sequencing to characterize cytochrome c oxidase I in amplicons from 20,000 unique arthropod specimens as part of the efforts to DNA fingerprint and register species to better understand biodiversity. This work revealed that through multiplexing of samples, the Sequel System enables sequencing of millions of samples per year. Explore this research further:

Hebert, P.D.N. et al., 2018. A Sequel to Sanger: amplicon sequencing that scales. BMC genomics, 19(1), p.219.

To learn more about how SMRT Sequencing can give you the long view of your targeted regions, contact us.

Selected Resources