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Thursday, September 29, 2016

West Coast User Group Meeting Recap:
SMRT Sequencing for Goats, Grapes, Personalized Medicine & More

More than 150 SMRT Sequencing users gathered at Stanford University for our annual West Coast User Meeting & workshops earlier this month. Many thanks to all the scientists who attended and shared their research. For anyone who couldn’t make it, we’ve included some highlights from each talk below (and links to download the full presentations when possible): The event began with Marty Badgett, our senior product manager for the Sequel System and the PacBio RS II, discussing recent technology updates. He presented the most recent results from the Sequel System highlighting resequencing and small and large genome applications. Specifically, two…

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Tuesday, September 27, 2016

Sequel System Data Release: Arabidopsis Dataset and Genome Assembly

Today we are pleased to release the first Arabidopsis thaliana (Ler-0) dataset and de novo genome assembly generated with the Sequel System, using two SMRT Cells and 12 hours of runtime. Only three years ago, we released our first genome assembly1 for Arabidopsis produced on the PacBio RS II using P4-C2 chemistry, 85 SMRT Cells and 255 hours of runtime. Four months later, we released a second Arabidopsis dataset1 using the improved P5-C3 chemistry, which reduced the number of SMRT Cells to 46 and runtime to 138 hours. We produced this Sequel dataset using our latest chemistry enhancements which significantly…

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Tuesday, September 20, 2016

UPDATED Data Release: Comprehensive Alzheimer Brain Isoform Sequencing (Iso-Seq) Dataset

NOTE: This blog was updated with the latest data from the Sequel II System on March 9, 2020. Alzheimer’s disease (AD) is a devastating neurodegenerative disease that affects ~44 million people worldwide, making it the most common form of dementia. Pathologically it is defined by severe neuronal loss, aggregation of amyloid β (Aβ) in extracellular senile plaques in the brain, and formation of intraneuronal neurofibrillary tangles consisting of hyperphosphorylated tau protein.  Studies looking into disease mechanism have shown that changes in gene expression due to alternative splicing likely contribute to the initiation and progression of AD. Hence, efforts have been…

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Tuesday, September 6, 2016

Avian Celebrity: Kākāpō Genomes Make Headlines

A recent cover story in New Zealand Geographic vividly details the efforts to sequence not just the kākāpō genome, but the genomes of every single living kākāpō. If you missed our earlier blog about this bird, the kākāpō is a member of the parrot family known for its unique attributes: it’s heavy, flightless, and mostly active at night. As author Rebekah White reports in “Decoding Kākāpō,” the remaining members of this species — about 125 of them — live on islands near New Zealand. White recounts how scientist Jason Howard, a member of Erich Jarvis’s lab at Duke, first became…

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Thursday, September 1, 2016

Network with the SMRT Sequencing Community at Our Upcoming Events

We’re lucky to have such a vibrant community of SMRT Sequencing users, and there’s nothing better than getting them together to share tips, exchange ideas, and develop new applications. These upcoming events will facilitate just that — and we hope you can join us!   West Coast User Group Meeting & Workshops September 7-8 Frances C. Arrillaga Alumni Center at Stanford University, Palo Alto, Calif. Our annual West Coast event taking place next week will include the usual day-long user group meeting as well as half-day workshops on bioinformatics and sample preparation, which will take place on September 7th at…

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