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Friday, February 27, 2015

AGBT 2015: PacBio Workshop Review & Recording

Our AGBT workshop attracted more than 500 attendees thanks to the high-profile speakers who shared their perspectives on human genomic research. Because of the exclusivity of AGBT, we decided to live-stream our workshop to reach the broader scientific community. Thanks to the the hundreds of people who tuned in to our live webcast from afar! Here are some highlights from the presentations and the recording of the workshop is at the bottom of this post. Our CEO, Mike Hunkapiller, started the session with a reflection on the 15-year anniversary of the announcements of the first human genomes, noting these efforts…

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Friday, February 27, 2015

AGBT Highlights, Day Two: Human Genomes, Variation, and the Rapidly Evolving Y Chromosome

The first full day of AGBT kicked off with a great talk from Evan Eichler from the University of Washington. Starting with the premise that characterizing genetic variation is key to understanding phenotypes, his presentation offered in-depth looks into human genome projects designed to fully represent data missed in existing assemblies and current whole genome sequencing studies. Eichler pointed out that short-read sequencing misses a lot of structural variation, particularly when it occurs near repeat-rich regions. He said that every genome sequenced with short-read technology is missing important variation, and that a big problem is our inability to quantify just…

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Thursday, February 26, 2015

AGBT Highlights, Day One: Advancing Human Reference Assembly & Sequencing in the Clinic

It is great to be here in Marco Island for the AGBT meeting! The 16th annual meeting hit the ground running with a pre-meeting workshop hosted by the Genome Reference Consortium (GRC) followed by an opening session that was more clinically focused than many attendees are used to at this tech-heavy conference. From the dynamic Q&A sessions, it was clear that these were precisely the kind of talks that people have been looking for as this meeting evolves downstream along with genomic science. The GRC workshop, entitled ‘Advancing the Human Reference Assembly’ included four speakers: Valerie Schneider (NCBI), Tina Graves-Lindsay…

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Tuesday, February 24, 2015

AGBT 2015: Seeing the Genome in a New Light (Sunshine?)

Like many others, we’re looking forward to an exciting week of science and sun at the 16th annual Advances in Genome Biology and Technology (AGBT) conference! We’re hosting a lunch workshop on Friday, February 27, in the Palms Ballroom from 12:00 pm to 2:00 pm EST. We hope you can join us onsite (please reserve your seat) and even if you’re not at the conference, you can watch the live stream. Here’s the agenda: Towards Comprehensive Genomics – Past, Present and Future The Human Genome: From One to One Million J. Craig Venter, Human Longevity Inc. Is Perfect Assembly Possible?…

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Wednesday, February 4, 2015

High-Quality Genome Assembly and Transcriptome of Cotton Using SMRT Sequencing

A recent research partnership with KeyGene, a Dutch plant genomics and crop improvement company, has resulted in an integrated whole-genome assembly and transcriptome of Gossypium hirsutum, or tetraploid cotton. This is the first known complete assembly for a polyploid crop with a genome larger than 2 Gb. KeyGene has a long established reputation for generating high-quality data even for very complex genomes. For this project, the cotton genome was sequenced with 38x coverage using Single-Molecule, Real-Time (SMRT®) Sequencing. Assembly of PacBio® long reads reduced the number of contigs from more than 1 million in an existing short-read assembly to fewer…

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