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Wednesday, January 29, 2014

At Plant & Animal Genome Workshop, Users Showcase Projects Enabled by SMRT Sequencing

Earlier this month, we hosted a workshop at the International Plant & Animal Genome (PAG) conference in San Diego entitled “A SMRT® Sequencing Approach to Reference Genomes, Annotation, and Haplotyping.” PacBio users presented data on various projects that have benefited from long-read sequence data, including several that had previously been attempted with short-read technologies without success. We were delighted to see reports on newer features of SMRT Sequencing, including full-length isoforms, automated haplotyping, and more. Here’s a recap, as well as links to video recordings of the presentations: Chongyuan Luo, a scientist from Joe Ecker’s lab at the Salk Institute…

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Thursday, January 23, 2014

PacBio Service Provider DNA Link Sees Soaring Global Demand for SMRT Sequencing

Since its founding in 2000, the service provider team at Korea-based DNA Link has sought to differentiate itself from other facilities by being an early adopter of new technologies. The company started during the height of the Human Genome Project and initially offered genotyping assays such as SNaPshot® and TaqMan®. DNA Link later adopted microarrays, and when the next-generation sequencing (NGS) wave hit, the scientists quickly embraced the technology. DNA Link scientists purchased their PacBio® system virtually as soon as it was commercially available. Today, customer demand for Single Molecule, Real-Time (SMRT®) Sequencing is soaring — making DNA Link’s expertise…

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Tuesday, January 21, 2014

Genome Research Paper: Resolve Complex Genomic Regions for a ‘Fraction of the Cost’ With SMRT Sequencing

A new Genome Research paper describes the application of Single Molecule, Real-Time (SMRT®) Sequencing to resolve repeat-heavy genomic regions in important reference genomes such as human and chimpanzee. In the process, the authors drew some important conclusions about cost, pooling, and coverage requirements for this type of work. “Reconstructing complex regions of genomes using long-read sequencing technology” comes from lead author John Huddleston and senior author Evan Eichler at the University of Washington, along with collaborators at Washington University, the University of Bari, Bilkent University, and Pacific Biosciences. In the paper, Eichler and his collaborators note the steep cost of…

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Thursday, January 16, 2014

Looking Ahead: The 2014 PacBio Technology Roadmap

By Jonas Korlach, Chief Scientific Officer 2013 was an eventful and exciting year for PacBio. As I described in the 2013 roadmap post a year ago, we have applied numerous improvements to SMRT® Sequencing, resulting in longer read lengths, greater sequencing throughput, new and improved data-analysis methods, and more efficient workflows. We are very pleased that these advances resulted in so many publications, conference presentations, and social media contributions, with the number of peer-reviewed scientific publications from the scientific community now exceeding 100. On behalf of all of us at Pacific Biosciences, I would like to express my heartfelt gratitude…

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Monday, January 13, 2014

Data Release: Preliminary de novo Haploid and Diploid Assemblies of Drosophila melanogaster

Model organisms such as yeast, Arabidopsis and Drosophila have been essential to progress in genetic and biomedical research for more than 100 years.  Model organisms are the best, fastest, most effective way to advance science especially when human experimentation may not be feasible. Numerous biological principles have been elucidated using model organisms, including Nobel-prize winning discoveries by Thomas Hunt Morgan that genes are carried on chromosomes; by Hermann Muller for the discovery that X-ray irradiation causes mutations; and by Edward B. Lewis, Christiane Nüsslein-Volhard, and Eric Wieschaus for their discoveries revealing the genetic control of early embryonic development – all…

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Wednesday, January 8, 2014

SMRT Sequencing for Plant and Animal Genomes: Learn More at PAG 2014

Many recent studies have demonstrated the use of Single Molecule, Real-Time (SMRT®) Sequencing for larger genomes, from complete reference genomes to de novo discovery of transcript isoforms. These advances include understanding genome complexity and variation and enabling improved leverage of haplotype information for biotechnology. Some of these efforts will be presented at the workshop we’re hosting at this year’s International Plant & Animal Genome (PAG) conference in San Diego. Sign up now to reserve your seat or receive the recording after the event.  Workshop details: A SMRT® Sequencing Approach to Reference Genomes, Annotation, and Haplotyping Tuesday, January 15 1:30 –…

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