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Monday, September 16, 2013

Genome Biology Paper Highlights Affordability and Scale of PacBio-Based Finished Microbial Genomes

A new paper released in Genome Biology on September 13 from lead author Sergey Koren at the National Biodefense Analysis and Countermeasures Center offers a thorough overview of SMRT® Sequencing for microbes, from per-genome cost to potential for assembling complete genomes. In “Reducing assembly complexity of microbial genomes with single-molecule sequencing,” Koren and co-authors consider microbial genome assembly, which evolved over time from the Sanger days of manually finished genomes to short-read sequencers that offered lots of sequence data but virtually no finished genomes. Today, that evolution has continued with SMRT® Sequencing, which allows for rapid and complete genome assembly.…

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Thursday, September 5, 2013

Next Week’s ICG Meeting to Include Several SMRT Sequencing Talks

The International Conference on Genomics in the Americas (ICG), organized by BGI and UC Davis, is taking place on Sept 12-13 in Sacramento, CA. One of the keynote presentations in the opening session comes from Nobel Laureate Richard Roberts, Chief Scientific Officer of New England Biolabs. Entitled “Bacterial Methylomes,” the talk will cover some recent work Roberts has done using SMRT® Sequencing to characterize the full, genome-wide methylation marks in various strains of microbes. This kind of work “has yielded a plethora of new and interesting results,” Roberts told BioMed Central recently. Later that day, Ken Dewar from McGill University…

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Tuesday, September 3, 2013

New Data Release: Arabidopsis Assembly Offers Glimpse of
De Novo SMRT Sequencing for Larger Genomes

Update 1/13/14: A new data release of Arabidopsis using P5-C3 chemistry is available Advances in our chemistries, throughput, and read length are pushing the envelope in the way we tackle larger genomes. We recently sequenced the Landsberg erecta ecotype (Ler-0) of Arabidopsis thaliana and produced a successful assembly solely using PacBio® data. The data set resulting from this sequencing effort and assembly using SMRT® Portal is now available via Devnet for anyone who wants to give it a test drive. A few stats on Arabidopsis and the assembly using PacBio sequence data: Genome size: 124.6 Mb GC content: 33.92% Raw…

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