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Monday, May 4, 2015

PAG Grant Winner: Rainforest Tree Homalanthus nutans to get the SMRT Treatment

We’re pleased to announce the winner of our recent “Most Interesting Genome in the World” grant competition. Congratulations to Jay Keasling and Jeff Wong at the University of California, Berkeley! The grant program, which was supported by co-sponsors Sage Science, Computomics, and the Arizona Genomics Institute, was very competitive with more than 250 submitted proposals. Keasling and Wong will be awarded SMRT® Sequencing — using up to 40 SMRT Cells with BluePippin™ DNA size selection — for Homalanthus nutans, a small rainforest tree that grows in Samoa. The plant is critical as the source of a natural product called prostratin,…

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Thursday, April 24, 2014

SMRT Sequencing of Chicken Heart Transcripts Yields New Genes and Isoforms

The Gallus gallus (common chicken) genome was initially published in 2004, but the latest RefSeq and Ensembl annotations remain incomplete. The chicken is an important model organism, especially for research on embryogenesis and heart development. In a new paper published in PLOS One, researchers representing the Cardiovascular Development Consortium of the Bench to Bassinet Program and Pacific Biosciences describe work to improve the chicken genome annotation using SMRT® DNA Sequencing. In “Long-Read Sequencing of Chicken Transcripts and Identification of New Transcript Isoforms,” the consortium describes how they used SMRT sequencing to generate full-length cDNA reads from embryonic chicken hearts, combined…

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