Gain confidence with the highest consensus accuracy available
Single Molecule, Real-Time (SMRT) Sequencing delivers exceptional sequencing results, exceeding 99.999% (Q50) consensus accuracy. With this unmatched accuracy, low sequencing-context bias, and accurate mapping of sequencing reads, SMRT Sequencing provides the information needed to confidently call and detect variants.
Consensus accuracy as a function of coverage
SMRT Sequencing accuracy is a function of coverage and chemistry. The random distribution of single-pass errors allows consensus accuracy to rapidly build with coverage, and can even lead to perfect consensus as shown above. The Sequel chemistry 2.0 estimates shown here are based on a bacterial genome.
Contact us for more information about incorporating SMRT Sequencing into your research efforts.
- Zhang, Huibin et al. (2016) Comprehensive mutagenesis of the fimS promoter regulatory switch reveals novel regulation of type 1 pili in uropathogenic Escherichia coli. Proceedings of the National Academy of Sciences
- Huang, Da Wei et al. (2016) Towards better precision medicine: PacBio Single-Molecule long reads resolve the interpretation of HIV drug resistant mutation profiles at explicit quasispecies (haplotype) level. Journal of Data Mining in Genomics & Proteomics
- Russo, Giancarlo et al. (2015) Highly sensitive, non-invasive detection of colorectal cancer mutations using single molecule, third generation sequencing. Applied & Translational Genomics
- Berlin, Konstantin et al. (2015) Assembling large genomes with single-molecule sequencing and locality-sensitive hashing. Nature Biotechnology
- Koren, Sergey et al. (2015) One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly. Current Opinion in Microbiology
- Huddleston, John et al. (2014) Reconstructing complex regions of genomes using long-read sequencing technology. Genome Research
- Koren, Sergey et al. (2013) Reducing assembly complexity of microbial genomes with single-molecule sequencing. Genome Biology
- Roberts, Richard J et al. (2013) The advantages of SMRT sequencing. Genome Biology
- Chin, Chen-Shan et al. (2013) Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nature Methods
- Hestand, Matthew S et al. (2016) Polymerase specific error rates and profiles identified by single molecule sequencing. Mutation Research
- Jonas Korlach et al. (2014) Returning to more finished genomes Genomics Data
- Giallonardo, Francesca Di et al. (2014) Full-length haplotype reconstruction to infer the structure of heterogeneous virus populations. Nucleic Acids Research
- Poster: Heiner, C. et al. (2016) WGS SMRT Sequencing of patient samples from a fecal microbiota transplant trial
- Poster: Smith, M.L. et al. (2016) An improved circular consensus algorithm with an application to detect HIV-1 Drug Resistance Associated Mutations (DRAMs)
- Poster: Sethuraman, Anand et al. (2016) Highly sensitive and cost-effective detection of somatic cancer variants using single-molecule, real-time sequencing
- Poster: Hepler, N. Lance et al. (2016) An improved circular consensus algorithm with an application to detection of HIV-1 Drug-Resistance Associated Mutations (DRAMs)
- Poster: Sethuraman, Anand et al. (2015) Analysis of full-length metagenomic 16S genes by Single Molecule, Real-Time Sequencing
- Poster: Russo, Giancarlo et al. (2014) Highly sensitive, non-invasive detection of colorectal cancer mutations using single molecule, third generation sequencing.
- Paxinos, Ellen (2016) AGBT Virtual Poster: An improved circular consensus algorithm with an application to detect HIV-1 Drug Resistance Associated Mutations (DRAMs)
- Ashley, Euan (2016) AGBT Roche and PacBio Workshop: Towards precision medicine
- Gyllensten, Ulf (2015) AGBT Virtual Poster: Clinical sequencing using Pacific Biosciences RS II for HLA typing and monitoring of drug resistance in chronic myeloid leukemia (CML)
- Korlach, Jonas (2016) Mendelspod: A home run on the first hit – PacBio’s Jonas Korlach
- Carneiro, Mauricio (2012) AGBT Virtual Poster: Evaluating the potential of new sequencing technologies for genotyping and variation discovery in human data
- Korlach, Jonas (2014) Seminar: Gain new insights in genome and transcriptome research with >10,000 bp reads