PacBio sequencing provided complete minicircle sequences which avoided the assembly problem of short reads caused by the conserved regions. Minicircles were identified by a characteristic size, the presence of three short conserved sequences, a region of inherently bent DNA and the presence of single gRNA genes at a fairly defined location.

---

The Sequel System delivers long reads, high consensus accuracy and uniform coverage, enabling more complete, accurate, and contiguous assemblies of these large complex genomes. The latest Sequel chemistry increases yield up to 8 Gb per SMRT Cell for long insert libraries >20 kb and up to 10 Gb per SMRT Cell for libraries >40 kb.

---

With Single Molecule, Real-Time (SMRT®) sequencing and the Sequel® II or Sequel IIe system, you can easily and affordably sequence complete transcript isoforms in genes of interest or across the entire transcriptome. The Iso-Seq® method allows users to generate full-length cDNA sequences up to 10 kb in length — with no assembly required ...

---

MENLO PARK, Calif., Sept. 27, 2022 /PRNewswire/ -- PacBio (NASDAQ: PACB), a leading developer of high-quality, highly accurate sequencing solutions, today announced the availability of a new computational analysis method for profiling more than a million tandem repeats (TRs) across the human genome using PacBio's native long-read HiFi ...

---

MENLO PARK, Calif., Oct. 11, 2023 /PRNewswire/ -- PacBio (NASDAQ: PACB), a leading developer of high-quality, highly accurate sequencing solutions, today announced the availability of PacBio WGS Variant Pipeline – a complete, standardized computational method for HiFi whole genome sequencing (WGS) data analysis.

---

complete closed genomes for most microbes. Complete plasmid sequences are often included as well. • The data analysis workflow is highly streamlined, with automated demultiplexing and simple push-button assembly. • In addition to a low number of contigs, assembly produces highly accurate consensus

---

Template Preparation Protocols. This guide describes an optimized procedure for preparing your DNA template for sequencing on the PacBio System. This Blunt-End Liga-tion protocol, is suitable for all libraries (greater than 250 bp amplicons) and offers a total preparation time of approximately four hours. SMRTbellTM Templates.

---

May 6, 2013 at 7:30 AM EDT. University of Washington) DNA sequencing. Published in the the paper by Chin et al. describes the hierarchical genome assembly process ( HGAP) and demonstrates the method for efficient, automated genome sequence results exceeding 99.999% (QV 50) accuracy.

---

The three-minute introductory video outlines the key advantages of SMRT sequencing: Long reads allow you to readily assemble complete genomes and sequence full-length transcripts. High accuracy provides over 99.99% accurate sequencing results.

---

solutions, today announced the availability of PacBio WGS Variant Pipeline a complete, standardized computational method for HiFi whole genome sequencing (WGS) data analysis. The new software pipeline will enable customers to resolve many different variant types, including single-nucleotide

---