+

X

Quality Statement

Pacific Biosciences is committed to providing high-quality products that meet customer expectations and comply with regulations. We will achieve these goals by adhering to and maintaining an effective quality-management system designed to ensure product quality, performance, and safety.

X

Image Use Agreement

By downloading, copying, or making any use of the images located on this website (“Site”) you acknowledge that you have read and understand, and agree to, the terms of this Image Usage Agreement, as well as the terms provided on the Legal Notices webpage, which together govern your use of the images as provided below. If you do not agree to such terms, do not download, copy or use the images in any way, unless you have written permission signed by an authorized Pacific Biosciences representative.

Subject to the terms of this Agreement and the terms provided on the Legal Notices webpage (to the extent they do not conflict with the terms of this Agreement), you may use the images on the Site solely for (a) editorial use by press and/or industry analysts, (b) in connection with a normal, peer-reviewed, scientific publication, book or presentation, or the like. You may not alter or modify any image, in whole or in part, for any reason. You may not use any image in a manner that misrepresents the associated Pacific Biosciences product, service or technology or any associated characteristics, data, or properties thereof. You also may not use any image in a manner that denotes some representation or warranty (express, implied or statutory) from Pacific Biosciences of the product, service or technology. The rights granted by this Agreement are personal to you and are not transferable by you to another party.

You, and not Pacific Biosciences, are responsible for your use of the images. You acknowledge and agree that any misuse of the images or breach of this Agreement will cause Pacific Biosciences irreparable harm. Pacific Biosciences is either an owner or licensee of the image, and not an agent for the owner. You agree to give Pacific Biosciences a credit line as follows: "Courtesy of Pacific Biosciences of California, Inc., Menlo Park, CA, USA" and also include any other credits or acknowledgments noted by Pacific Biosciences. You must include any copyright notice originally included with the images on all copies.

IMAGES ARE PROVIDED BY Pacific Biosciences ON AN "AS-IS" BASIS. Pacific Biosciences DISCLAIMS ALL REPRESENTATIONS AND WARRANTIES, EXPRESS, IMPLIED OR STATUTORY, INCLUDING, BUT NOT LIMITED TO, NON-INFRINGEMENT, OWNERSHIP, MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL Pacific Biosciences BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, PUNITIVE, OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER WITH RESPECT TO THE IMAGES.

You agree that Pacific Biosciences may terminate your access to and use of the images located on the PacificBiosciences.com website at any time and without prior notice, if it considers you to have violated any of the terms of this Image Use Agreement. You agree to indemnify, defend and hold harmless Pacific Biosciences, its officers, directors, employees, agents, licensors, suppliers and any third party information providers to the Site from and against all losses, expenses, damages and costs, including reasonable attorneys' fees, resulting from any violation by you of the terms of this Image Use Agreement or Pacific Biosciences' termination of your access to or use of the Site. Termination will not affect Pacific Biosciences’ rights or your obligations which accrued before the termination.

I have read and understand, and agree to, the Image Usage Agreement.

I disagree and would like to return to the Pacific Biosciences home page.

Pacific Biosciences
Contact:

Sample and Preparation Kits for the Sequel System

 

 

SMRTbell Template Prep Kit 1.0 – SPv3

100-991-900

The SMRTbell Template Prep Kit 1.0 – SPv3 contains reagent supplies to perform SMRTbell library preparations of primer-annealed, symmetric SMRTbell libraries for insert sizes ranging from 500 bp to over 20 kb. Reagent quantities support 10 library preparations. SMRTbell Template Prep Kit 1.0 – SPv3 is a bundle of 2 kits: the SMRTbell Template Prep Kit 1.0 and Sequencing Primer v3. This kit includes both Sequencing Primer v2 for use with Sequel Sequencing Kit 1.2 and Sequencing Primer v3 for use with Sequel Sequencing Kits 1.2.1 and 2.0. Each primer supports 10 primer annealing reactions.

For bulk packaging, please contact your local field application scientist or sales representative.

The following is a sample of applicable protocols. For a complete list, please visit documentation and search for a protocol applicable to your research.

Procedure & Checklist: 20 kb Template Preparation Using BluePippin Size-Selection System for Sequel Systems

Procedure & Checklist: Preparing Greater than 30 kb SMRTbell Libraries Using Megaruptor Shearing

SMRTbell Damage Repair Kit – SPv3

100-992-200
The SMRTbell Damage Repair Kit – SPv3 contains reagent supplies to perform DNA Damage Repair, Exonuclease III and VII treatments, and primer- annealing of SMRTbell libraries for insert sizes ranging from 500 bp to over 20 kb. Reagent quantities support 10 library preparations. To be used with SMRTbell Barcoded Adapter Complete Prep Kit to generate primer-annealed SMRTbell libraries ready for polymerase binding or to be used independently for extra DNA Damage Repair of template. This kit includes both Sequencing Primer v2 for use with PacBio RS II and Sequencing Primer v3 for use with Sequel System. Each primer supports 10 primer annealing reactions.

SMRTbell Barcoded Adapter Complete Prep Kit – 96

100-514-900
The SMRTbell Barcoded Adapter Complete Prep Kit – 96 contains both the SMRTbell Barcoded Adapter Prep Kit and Barcoded Adapter Plate – 96. Reagent quantities support 96 individual barcoded adapter end-repair and ligation reactions. For use with the SMRTbell Damage Repair Kit to prepare primer-annealed SMRTbell libraries.

SMRTbell Barcoded Adapter Prep Kit

100-465-800
The SMRTbell Barcoded Adapter Prep Kit contains reagent supplies for the End Repair of templates and Ligation of SMRTbell barcoded adapters. Reagent quantities support 96 reactions.

Barcoded Adapter Plate – 96

Barcoded Universal F/R Primer Plate – 96

100-466-100
The Barcoded Universal F/R Primer Plate – 96 contains both forward and reverse primers to be used in a PCR reaction to add barcodes to PCR amplicons that have been amplified with universal sequence tags. Each of the 96 wells contains a different barcode and sufficient reagents for a single use. For use in conjunction with the SMRTbell Template Prep kit.

AMPure PB Beads

100-265-900
AMPure PB magnetic beads are specifically formulated for the SMRT Sequencing workflow. Beads are used for DNA cleanup and purification during sample extraction and at various stages in the workflow.

The following is a sample of applicable protocols. For a complete list, please visit documentation and search for a protocol applicable to your research.

Procedure & Checklist: 500bp Template Preparation and Sequencing

Procedure & Checklist: 20 kb Template Preparation Using BluePippin Size-Selection

 

For more information about how our Sequel System consumables can support your research, contact us.

Event

Dutch Society of Human Genetics Meeting (NVHG) 2017

September 21 - September 22, 2017

Stay
Current

Visit our blog »