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Sample and Preparation Kits for the Sequel System

 

SMRTbell Template Prep Kit 1.0

100-259-100
The SMRTbell Template Prep Kit 1.0 contains reagent supplies to perform SMRTbell library preparations of non-size-selected or size-selected, primer-annealed SMRTbell libraries for insert sizes ranging from 500 bp to greater than 20 kb. Reagent quantities support 10 library preparations.

The following is a sample of applicable protocols. For a complete list, please visit documentation and search for a protocol applicable to your research.

Procedure & Checklist: 500 bp Template Preparation and Sequencing

Procedure & Checklist: 20 kb Template Presentation Using BluePippin Size-Selection

SMRTbell HT (High-Throughput) Template Prep Kit

100-286-300

The SMRTbell HT Template Prep Kit contains reagent supplies to perform 96 automated SMRTbell library preparations of non-size-selected, primer-annealed SMRTbell libraries for insert sizes ranging from 500 bp to greater than 20 kb. Reagents come in 4 x 24 reaction sets. The reagents in the SMRTbell HT Template Prep Kit are found in different volume portions from the SMRTbell Template Prep Kit 1.0 and are not scalable from one to another.

The reagents are compatible with protocols that have been co-developed for the Agilent NGS Workstation Option B and the PerkinElmer Sciclone NGSx Workstation. The available protocols for these systems have only been validated with the PacBio 10 kb template preparation workflow.

To learn more, please contact your local field application scientist (FAS) or sales representative.

Not applicable

SMRTbell Barcoded Adapter Complete Prep Kit-96

100-514-900
The SMRTbell Barcoded Adapter Complete Prep Kit-96 contains both the SMRTbell Barcoded Adapter Prep Kit and Barcoded Adapter Plate-96. Reagent quantities support 96 individual barcoded adapter end-repair and ligation reactions. For use with the SMRTbell Damage Repair Kit to prepare primer-annealed SMRTbell libraries.

SMRTbell Damage Repair Kit

100-465-900
The SMRTbell Damage Repair Kit contains reagents to perform DNA damage repair and anneal sequencing primers. The SMRTbell Damage Repair Kit is used with the SMRTbell Barcoded Adapter Complete Prep Kit-96 to complete the SMRTbell library workflow for samples using barcoded adapters. Additionally, the SMRTbell Damage Repair Kit can be used as a standalone DNA damage-repair reaction where applicable. Reagent quantities support 10 preparations.

Barcoded Universal F/R Primer Plate-96

100-466-100
The Barcoded Universal F/R Primer Plate-96 contains both forward and reverse primers to be used in a PCR reaction to add barcodes to PCR amplicons that have been amplified with universal sequence tags. Each of the 96 wells contains a different barcode and sufficient reagents for a single use. For use in conjunction with the SMRTbell Template Prep kit.

AMPure PB Beads

100-265-900
AMPure PB magnetic beads are specifically formulated for the SMRT Sequencing workflow. Beads are used for DNA cleanup and purification during sample extraction and at various stages in the workflow.

The following is a sample of applicable protocols. For a complete list, please visit documentation and search for a protocol applicable to your research.

Procedure & Checklist: 500bp Template Preparation and Sequencing

Procedure & Checklist: 20 kb Template Preparation Using BluePippin Size-Selection

 

For more information about how our Sequel System consumables can support your research, contact us.

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