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Expand your multiplexing efforts

PacBio Systems provide the ability to sequence multiple samples on the same SMRT Cell to increase throughput and enhance the efficiency of your research.

 

For information on barcoding options including protocols and analysis, please refer to the information in the Barcoding Overview.

 

Sequence files (FASTA1) with PacBio barcodes for use on the Sequel System and the PacBio RS II:

1These are FASTA files saved with .docx extension. To be able to use them once you download the file replace the .docx extension with .fasta.

 

Sequence files (FASTA1) with PacBio barcodes for use on the PacBio RS II:

  • 96 barcodes for use with Barcoded Primers
  • 384 barcodes for use with Barcoded Primers
  • 96 barcodes for use with the Barcoded Universal F/R Primers Plate – 96 (PacBio Product
    Part Number: 100-466-100)
  • 96 barcodes for use with the Barcoded Adapter Complete Prep Kit – 96 (PacBio Product
    Part Number: 100-514-900)

1These are FASTA files saved with .docx extension. To be able to use them once you download the file replace the .docx extension with .fasta.

 

Map of the old PacBio RS II barcode names to the new Sequel System and PacBio RS II

 

Note: The Iso-Seq application supports a different multiplexing strategy. For details about barcoding samples for Iso-Seq analysis, see Barcoding Samples for Isoform Sequencing (Iso-Seq Analysis).

 

Barcode oligo ordering sheets:

Sample prep protocols for multiplexed experiments:

Contact us to learn more about how this innovative software can support your research efforts.