+

X

Quality Statement

Pacific Biosciences is committed to providing high-quality products that meet customer expectations and comply with regulations. We will achieve these goals by adhering to and maintaining an effective quality-management system designed to ensure product quality, performance, and safety.

X

Image Use Agreement

By downloading, copying, or making any use of the images located on this website (“Site”) you acknowledge that you have read and understand, and agree to, the terms of this Image Usage Agreement, as well as the terms provided on the Legal Notices webpage, which together govern your use of the images as provided below. If you do not agree to such terms, do not download, copy or use the images in any way, unless you have written permission signed by an authorized Pacific Biosciences representative.

Subject to the terms of this Agreement and the terms provided on the Legal Notices webpage (to the extent they do not conflict with the terms of this Agreement), you may use the images on the Site solely for (a) editorial use by press and/or industry analysts, (b) in connection with a normal, peer-reviewed, scientific publication, book or presentation, or the like. You may not alter or modify any image, in whole or in part, for any reason. You may not use any image in a manner that misrepresents the associated Pacific Biosciences product, service or technology or any associated characteristics, data, or properties thereof. You also may not use any image in a manner that denotes some representation or warranty (express, implied or statutory) from Pacific Biosciences of the product, service or technology. The rights granted by this Agreement are personal to you and are not transferable by you to another party.

You, and not Pacific Biosciences, are responsible for your use of the images. You acknowledge and agree that any misuse of the images or breach of this Agreement will cause Pacific Biosciences irreparable harm. Pacific Biosciences is either an owner or licensee of the image, and not an agent for the owner. You agree to give Pacific Biosciences a credit line as follows: "Courtesy of Pacific Biosciences of California, Inc., Menlo Park, CA, USA" and also include any other credits or acknowledgments noted by Pacific Biosciences. You must include any copyright notice originally included with the images on all copies.

IMAGES ARE PROVIDED BY Pacific Biosciences ON AN "AS-IS" BASIS. Pacific Biosciences DISCLAIMS ALL REPRESENTATIONS AND WARRANTIES, EXPRESS, IMPLIED OR STATUTORY, INCLUDING, BUT NOT LIMITED TO, NON-INFRINGEMENT, OWNERSHIP, MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL Pacific Biosciences BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, PUNITIVE, OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER WITH RESPECT TO THE IMAGES.

You agree that Pacific Biosciences may terminate your access to and use of the images located on the PacificBiosciences.com website at any time and without prior notice, if it considers you to have violated any of the terms of this Image Use Agreement. You agree to indemnify, defend and hold harmless Pacific Biosciences, its officers, directors, employees, agents, licensors, suppliers and any third party information providers to the Site from and against all losses, expenses, damages and costs, including reasonable attorneys' fees, resulting from any violation by you of the terms of this Image Use Agreement or Pacific Biosciences' termination of your access to or use of the Site. Termination will not affect Pacific Biosciences’ rights or your obligations which accrued before the termination.

I have read and understand, and agree to, the Image Usage Agreement.

I disagree and would like to return to the Pacific Biosciences home page.

Pacific Biosciences
Contact:

Expand your multiplexing efforts

Selected applications on PacBio Systems provide the ability to sequence multiple samples on the same SMRT Cell.

Scoring modes

SMRT Analysis barcoding protocol includes two scoring modes, which can be used according to study design and needs. The scoring modes are determined by understanding whether the barcode sequences on either end of the insert are the same or different.

  • Symmetric Mode: Barcode sequences are the same on both sides of the insert.
  • Asymmetric Mode: Different barcode sequences on either end of the insert (a.k.a. ‘paired’ mode).

Learn more about the barcoding protocols and their scoring modes.

SMRT Analysis applications supporting multiplexing

SMRT Portal includes four protocols that make use of the barcoding module for demultiplexing data sets and basic filtering functionality. It also ensures high-fidelity binning of outputs. For more fine-tuned filtering, see some command-line options.

RS_Subreads

Generate barcode scoring file and export one fastq file per barcode.

RS_ReadsOfInsert

Generalized Circular Consensus Sequencing (CCS) protocol. Use this protocol for single-molecule consensus analysis with barcoding.

RS_Resequencing_Barcode

Generate alignments labeled by barcode.

Learn more about per-barcode variant calls using Quiver and/or MinorVariants.

RS_Long_Amplicon_Analysis

Finds phased consensus sequences from a pooled set of polyploid amplicons.

Additional resources

  1. Software brochure
  1. SMRT Analysis training

 

Contact us to learn more about how this innovative software can support your research efforts.

Event

Precision Medicine World Conference (PMWC 2017)

January 23 - January 25, 2017

Stay
Current

Visit our blog »