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お問い合わせ

ロングリードで比類ない結果を産出

ゲノミクスの分野が進展するにつれ、科学者コミュニティではロングリードの重要性が高まってきています。ロングリードはリシークエンスのマッピングを向上し、de novoアセンブリをより簡単に実施します。PacBioシステムではDNAを直接シークエンスし、均一なゲノムカバレッジをもたらすロングリードを手にすることができます。1分子リアルタイム(SMRT)シークエンス技術により、業界でも屈指のロングリード(平均:10 kb、長いリードは60 kbを到達)を研究に利用できます。

ロングリードが可能にするアプリケーション

  • 高品質de novoアセンブリでゲノム配列を構築
  • 完全長アイソフォームのカタログ化
  • シーケンスマッピングにおける曖昧さの解決
  • 完全にフェーズ化されたアリルの観察
  • リピートおよび複雑な領域をシークエンス
  • 構造多型を解明

SMRT_Technology_Read_Lengths.Spotlight.Read_length
Sequelシステム(10時間ムービーラン、2.0ケミストリー)におけるリード長データ。30 kbにサイズ選択したライブラリーを使用し、データ合計は7.6 Gb。Sequelシステムの各SMRT Cell 1Mからは約365,000リードを産出。

SMRTシークエンスをどのように研究に応用できるか、ぜひお問い合わせください。

 

Selected Resources