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Quality Statement

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Image Use Agreement

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ターゲット領域をシークエンス

ターゲットシークエンスは多型や変異の探索および検出をコスト効率よく行える強力な手法です。関連領域の包括的な像を捉えるには、興味領域を均一に、かつ完全にカバーするシークエンスデータが必要です。現在、ショートリードシークエンサーではミスアライメントが生じたり、リピート領域を網羅することが難しい状況です。さらにGCリッチな領域では、PCRバイアスがバリアントコールに不十分なカバレッジをもたらすことが報告されています1

包括的な遺伝的多型および変異へのアクセス

1分子リアルタイム(SMRT) シークエンスは、1分子単位でのシークエンス結果、ロングリード、そして複雑なゲノムに対して最も低いバイアスを誇ります。これにより、構造多型、稀なSNPやindel、コピー数多型、マイクロサテライト、ハプロタイプ、そしてフェージングなどの解析が明確に行えます。PacBioシステムを用いたターゲットシークエンスにより、SNPのカタログ化にとどまらず、リピート伸長やプロモーター、トランスポゾン因子の近隣陵域においてもすべての種類の多型や変異にアクセスできます。

 

PacBioシークエンスで他の技術が見逃したターゲットを捉える

SMRTシークエンスを用いた包括的な遺伝的多型の解析については、ぜひお問い合わせください。

 

 References

  1. Carneiro, M. O. et al., (2012) Pacific Biosciences sequencing technology for genotyping and variation discovery in human data. BMC Genomics. 13, 375.

Selected Resources