+

X

Quality Statement

Pacific Biosciences is committed to providing high-quality products that meet customer expectations and comply with regulations. We will achieve these goals by adhering to and maintaining an effective quality-management system designed to ensure product quality, performance, and safety.

X

Image Use Agreement

By downloading, copying, or making any use of the images located on this website (“Site”) you acknowledge that you have read and understand, and agree to, the terms of this Image Usage Agreement, as well as the terms provided on the Legal Notices webpage, which together govern your use of the images as provided below. If you do not agree to such terms, do not download, copy or use the images in any way, unless you have written permission signed by an authorized Pacific Biosciences representative.

Subject to the terms of this Agreement and the terms provided on the Legal Notices webpage (to the extent they do not conflict with the terms of this Agreement), you may use the images on the Site solely for (a) editorial use by press and/or industry analysts, (b) in connection with a normal, peer-reviewed, scientific publication, book or presentation, or the like. You may not alter or modify any image, in whole or in part, for any reason. You may not use any image in a manner that misrepresents the associated Pacific Biosciences product, service or technology or any associated characteristics, data, or properties thereof. You also may not use any image in a manner that denotes some representation or warranty (express, implied or statutory) from Pacific Biosciences of the product, service or technology. The rights granted by this Agreement are personal to you and are not transferable by you to another party.

You, and not Pacific Biosciences, are responsible for your use of the images. You acknowledge and agree that any misuse of the images or breach of this Agreement will cause Pacific Biosciences irreparable harm. Pacific Biosciences is either an owner or licensee of the image, and not an agent for the owner. You agree to give Pacific Biosciences a credit line as follows: "Courtesy of Pacific Biosciences of California, Inc., Menlo Park, CA, USA" and also include any other credits or acknowledgments noted by Pacific Biosciences. You must include any copyright notice originally included with the images on all copies.

IMAGES ARE PROVIDED BY Pacific Biosciences ON AN "AS-IS" BASIS. Pacific Biosciences DISCLAIMS ALL REPRESENTATIONS AND WARRANTIES, EXPRESS, IMPLIED OR STATUTORY, INCLUDING, BUT NOT LIMITED TO, NON-INFRINGEMENT, OWNERSHIP, MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL Pacific Biosciences BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, PUNITIVE, OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER WITH RESPECT TO THE IMAGES.

You agree that Pacific Biosciences may terminate your access to and use of the images located on the PacificBiosciences.com website at any time and without prior notice, if it considers you to have violated any of the terms of this Image Use Agreement. You agree to indemnify, defend and hold harmless Pacific Biosciences, its officers, directors, employees, agents, licensors, suppliers and any third party information providers to the Site from and against all losses, expenses, damages and costs, including reasonable attorneys' fees, resulting from any violation by you of the terms of this Image Use Agreement or Pacific Biosciences' termination of your access to or use of the Site. Termination will not affect Pacific Biosciences’ rights or your obligations which accrued before the termination.

I have read and understand, and agree to, the Image Usage Agreement.

I disagree and would like to return to the Pacific Biosciences home page.

Pacific Biosciences
Contact:

Expand the scope of epigenetic investigation

DNA methylation significantly impacts many biological processes, including the microbial cell cycle, gene expression, and DNA repair. It has also been shown to be a critical factor in virulence and disease1. Even though thousands of methyltransferase enzymes have been recorded in the REBASE database, epigenetic investigations have been restricted by limited indirect detection methods, leaving many DNA modifications and methyltransferase specificities uncharacterized.

Discover epigenetic modifications with direct detection

Single Molecule, Real-Time (SMRT) Sequencing offers direct detection of DNA modifications by measuring variation in the polymerase kinetics of DNA base incorporation during sequencing. The PacBio Systems have the sensitivity to detect epigenetic modification without amplification or chemical conversions. These enhanced capabilities support hypothesis-free analysis of sequences for epigenetic signatures, previously unknown DNA modifications, and methyltransferase recognition motifs throughout bacterial genomes. With SMRT Sequencing, you have the ability to:

  • Simultaneously detect multiple types of epigenetic modifications
  • Visualize genome-wide methylation with motif analysis at single-base and DNA-strand resolution
  • Obtain complete genomes with annotations for epigenetic modification
  • Resolve and phase strand-specific modifications

spotlight-microbiology-icon

Workflow: from DNA to microbial methylome

  • SMRT Sequencing with PacBio Systems
    • Achieve average read lengths of ~10 kb
    • Complete microbial genomes in a single SMRT Cell on the PacBio RS II

Featured research: Streptococcus pneumonia switches pathogenicity based on epigenetic modification

Microbial_Epigenetics.Spotlight2.Streptococcus_pneumoniae_epigenetic_modification

Streptococcus pneumoniae, one of the world’s foremost bacterial pathogens, switches between phenotypic forms, or phases, that favor either asymptomatic carriage or invasive disease. Scientists used SMRT Sequencing genome and methylome analysis to prove the underlying mechanism for this switch depends on epigenetic regulation of a Type I restriction-modification system (SpnD39III). Each of these six phase variants has a differential effect on immunoevasion and virulence2.

Explore this research further.

To learn more about how you can use SMRT Sequencing for microbial epigenetics, contact us.


References

  1. Davis, B. M., et al., (2013) Entering the era of bacterial epigenomics with single molecule real time DNA sequencing. Current Opinion in Microbiology. 16 (2), 192–198.
  2. Manso, A.S., et al., (2014) A random six-phase switch regulates pneumococcal virulence via global epigenetic changes. Nature Communications. 5, 5055.

 

Selected Resources