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Resolving complexity

Advanced exploration of microbial communities requires improved approaches. Long-read sequencing enables species-level resolution and comprehensive characterization, from full-length 16S rRNA to shotgun metagenomics, for complete genome assemblies that drive functional insights.

Exploring form and function

Single Molecule, Real-Time (SMRT) Sequencing using the PacBio Systems provides long reads, high accuracy, and single-molecule resolution enabling you to:

  • Identify full-length genes using long-read metagenomic profiling – no assembly required
  • Achieve deeper strain-level resolution of community composition and phylogeny
  • Reconstruct longer contigs in whole-genome shotgun metagenomic assemblies and discover novel genes and gene clusters
  • Leverage epigenomic data to associate contigs and plasmids from closely related strains

Workflows: from DNA to resolved microbial communities

Learn more about these workflows here

 

Library preparation

SMRT Sequencing with the Sequel System

  • Achieve ~10 kb average read lengths, with some reads as long as 60 kb
  • Scalable throughput based on project needs
    • 100-fold coverage to resolve 16S rRNA sequences
    • 50-fold coverage per genome for de novo metagenomic assembly

Data analysis tools available through SMRT Analysis or PacBio DevNet

Spotlight: Accurately mapping mycobiota to understand human health

Scientists explored various sequencing techniques to establish the most rigorous method to map human gut mycobiota of healthy individuals. Their results showed that PacBio SMRT Sequencing most accurately represented the metagenomic population profile. Explore this research further:

Motooka, D. et al., 2017. Fungal ITS1 deep-sequencing strategies to reconstruct the composition of a 26-species community and evaluation of the gut mycobiota of healthy Japanese individuals. Frontiers in Microbiology, 8, p.238.

To learn more about how to fully resolve your microbial community, contact us.

Selected Resources